Selective Gene Expression by Postnatal Electroporation during Olfactory Interneuron Nurogenesis.

Selective Gene Expression by Postnatal Electroporation during Olfactory Interneuron Nurogenesis. Research Abstract Details 

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Selective Gene Expression by Postnatal Electroporation during Olfactory Interneuron Nurogenesis. Abstract Text:

Alexander T Chesler,Claire E Le Pichon,Jessica H Brann,Ricardo C Araneda,Dong-Jing Zou,Stuart Firestein,

Neurogenesis persists in the olfactory system throughout life. The mechanisms of how new neurons are generated, how they integrate into circuits, and their role in coding remain mysteries. Here we report a technique that will greatly facilitate research into these questions. We found that electroporation can be used to robustly and selectively label progenitors in the Subventicular Zone. The approach was performed postnatally, without surgery, and with near 100% success rates. Labeling was found in all classes of interneurons in the olfactory bulb, persisted to adulthood and had no adverse effects. The broad utility of electroporation was demonstrated by encoding a calcium sensor and markers of intracellular organelles. The approach was found to be effective in wildtype and transgenic mice as well as rats. Given its versatility, robustness, and both time and cost effectiveness, this method offers a powerful new way to use genetic manipulation to understand adult neurogenesis.

Selective Gene Expression by Postnatal Electroporation during Olfactory Interneuron Nurogenesis. Publishing Authors By Initials

AT Chesler,CE Le Pichon,JH Brann,RC Araneda,DJ Zou,S Firestein,

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Selective Gene Expression by Postnatal Electroporation during Olfactory Interneuron Nurogenesis. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: PLoS ONE

VOLUME: 3

Page Numbers: e1517

Journal Abbreviation: PLoS ONE

ISSN: 1932-6203

DAY: 30

MONTH: 01

YEAR: 2008

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LANGUAGE: eng

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AFFILIATION: Department of Biological Sciences, Columbia University, New York, New York, United States of America.

Country: United States

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