Selective encapsulation of single cells and subcellular organelles into picoliter- and femtoliter-volume droplets.

Selective encapsulation of single cells and subcellular organelles into picoliter- and femtoliter-volume droplets. Research Abstract Details 

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Selective encapsulation of single cells and subcellular organelles into picoliter- and femtoliter-volume droplets. Abstract Text:

Mingyan He,J Scott Edgar,Gavin D M Jeffries,Robert M Lorenz,J Patrick Shelby,Daniel T Chiu,

This paper describes a method, which combines optical trapping and microfluidic-based droplet generation, for selectively and controllably encapsulating a single target cell or subcellular structure, such as a mitochondrion, into a picoliter- or femtoliter-volume aqueous droplet that is surrounded by an immiscible phase. Once the selected cell or organelle is encased within the droplet, it is stably confined in the droplet and cannot be removed. We demonstrate in droplet the rapid laser photolysis of the single cell, which essentially "freezes" the state that the cell was in at the moment of photolysis and confines the lysate within the small volume of the droplet. Using fluorescein di-beta-d-galactopyranoside, which is a fluorogenic substrate for the intracellular enzyme beta-galactosidase, we also assayed the activity of this enzyme from a single cell following the laser-induced lysis of the cell. This ability to entrap individual selected cells or subcellular organelles should open new possibilities for carrying out single-cell studies and single-organelle measurements.

Selective encapsulation of single cells and subcellular organelles into picoliter- and femtoliter-volume droplets. Publishing Authors By Initials

M He,JS Edgar,GD Jeffries,RM Lorenz,JP Shelby,DT Chiu,

For similar enzymes and coenzymes: enzymes: hydrolases: glycoside hydrolases: galactosidases: beta-galactosidase research abstracts see: enzymes and coenzymes: enzymes: hydrolases: glycoside hydrolases: galactosidases: beta-galactosidase research

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Selective encapsulation of single cells and subcellular organelles into picoliter- and femtoliter-volume droplets. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Analytical chemistry

VOLUME: 77

Page Numbers: 1539-44

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 15

MONTH: Mar

YEAR: 2005

Selective encapsulation of single cells and subcellular organelles into picoliter- and femtoliter-volume droplets. Information

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LANGUAGE: eng

NlmUniqueID: 370536

Selective encapsulation of single cells and subcellular organelles into picoliter- and femtoliter-volume droplets. Keywords Mesh Terms:

KEYWORDS: beta-Galactosidase

MESH TERMS: metabolism

Chemical & Substance for Abstract: Selective encapsulation of single cells and subcellular organelles into picoliter- and femtoliter-volume droplets. Information

Substance Name: beta-Galactosidase

Registry Number: EC 3.2.1.23

Grant and Affiliation Information for Selective encapsulation of single cells and subcellular organelles into picoliter- and femtoliter-volume droplets.

AFFILIATION: Department of Chemistry, University of Washington, Seattle, Washington 98195-1700, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM65293-01

ACRONYM: GM

MEDLINETA: Anal Chem

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