Selection of smart aptamers by equilibrium capillary electrophoresis of equilibrium mixtures (ECEEM).

Selection of smart aptamers by equilibrium capillary electrophoresis of equilibrium mixtures (ECEEM). Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Selection of smart aptamers by equilibrium capillary electrophoresis of equilibrium mixtures (ECEEM). Abstract Text:

Andrei Drabovich,Maxim Berezovski,Sergey N Krylov,

We coin a term of "smart aptamers", which describes aptamers with predefined binding parameters of their interaction with the target. Here, we introduce a method for selection of smart aptamers with predefined values of Kd: equilibrium capillary electrophoresis of equilibrium mixtures (ECEEM). Conceptually, a mixture of a target with a DNA (RNA) library is prepared and equilibrated. A plug of the equilibrium mixture is injected into a capillary prefilled with a run buffer containing the target at the concentration identical to the target concentration in the equilibrium mixture. The components of the equilibrium mixture are separated by capillary electrophoresis while equilibrium is maintained between the target and aptamers. The unique feature of ECEEM is that aptamers with different Kd values migrate with different and predictable mobilities. Thus, collecting fractions with different mobilities results in smart aptamers with different and predefined Kd values. In this proof-of-principle work, we used ECEEM to select smart aptamers for MutS protein, for which aptamers have never been previously selected. Three rounds of ECEEM-based selection were sufficient to obtain smart aptamers with Kd values approaching theoretically predicted ones. ECEEM is the first method for aptamer selection whose ability to generate smart aptamers has been experimentally proven.

Selection of smart aptamers by equilibrium capillary electrophoresis of equilibrium mixtures (ECEEM). Publishing Authors By Initials

A Drabovich,M Berezovski,SN Krylov,

For similar nucleic acids, nucleotides, and nucleosides: nucleic acids: rna research abstracts see: nucleic acids, nucleotides, and nucleosides: nucleic acids: rna research

PUBMED ID PMID:

MEDLINE DATE:

Selection of smart aptamers by equilibrium capillary electrophoresis of equilibrium mixtures (ECEEM). Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 127

Page Numbers: 11224-5

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 17

MONTH: Aug

YEAR: 2005

Selection of smart aptamers by equilibrium capillary electrophoresis of equilibrium mixtures (ECEEM). Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

Selection of smart aptamers by equilibrium capillary electrophoresis of equilibrium mixtures (ECEEM). Keywords Mesh Terms:

KEYWORDS: RNA

MESH TERMS: chemistry

Chemical & Substance for Abstract: Selection of smart aptamers by equilibrium capillary electrophoresis of equilibrium mixtures (ECEEM). Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Selection of smart aptamers by equilibrium capillary electrophoresis of equilibrium mixtures (ECEEM).

AFFILIATION: Department of Chemistry, York University, Toronto, Ontario M3J 1P3, Canada.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Am Chem Soc

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Selection of smart aptamers by equilibrium capillary electrophoresis of equilibrium mixtures ECEEM Related Publications

• Aptamer-facilitated biomarker discovery (AptaBiD).
• Clinical Evaluation of Non-narcotic Substitutive Effects Produced by ANAR during the Therapy of Patients with Heroin Abuse.
• Dyson orbitals for ionization from the ground and electronically excited states within equation-of-motion coupled-cluster formalism: Theory, implementation, and examples.
• Equation-of-Motion Coupled-Cluster Methods for Open-Shell and Electronically Excited Species: The Hitchhiker's Guide to Fock Space.
• Hologram recording in DuPont photopolymer films by use of pulse exposure.
• Non-SELEX selection of aptamers.
• Nonequilibrium capillary electrophoresis of equilibrium mixtures--a single experiment reveals equilibrium and kinetic parameters of protein-DNA interactions.
• Psychotropic activity of the antialcohol preparation proproten-100.
• Radiation efficiency of natural modes of plates with beam stiffeners.
• Selection of smart aptamers by equilibrium capillary electrophoresis of equilibrium mixtures (ECEEM).
• Sweeping capillary electrophoresis: a non-stopped-flow method for measuring bimolecular rate constant of complex formation between protein and DNA.
• The theoretical prediction of infrared spectra of trans- and cis-hydroxycarbene calculated using full dimensional ab initio potential energy and dipole moment surfaces.
• Vibronic structure and ion core interactions in Rydberg States of diazomethane: an experimental and theoretical investigation.
• [A CASE OF RETICULOSIS WITH PREDOMINANT AFFECTION OF THE GASTROINTESTINAL TRACT AND WITH A PECULIAR HEMATOLOGIC PICTURE.]
• [Arthrosis and osteochondrosis]
• [Assessing the coccidiostatic properties of drugs]
• [Coccidiostatic effect of some chemotherapeutic compounds]
• [EFFECT OF CORTISONE ON THE DISTRIBUTION OF SULFANILAMIDE PREPARATIONS IN THE WHITE RAT ORGANISM DURING THE COURSE OF SERIAL THERAPY.]
• [Eating behavior disorders in neurotic patients]
• [Eimeria tenella resistance to coccidiovit]
• [Experience in the treatment of trichomoniasis and endometritis in cows]
• [Isolation of cultures of the causative agent of vibriosis by the membrane filter method]
• [Paclitaxel-eluting stents in treatment of stenoses and chronic occlusions of coronary arteries.]
• [Physio- and balneotherapy for diabetes mellitus patients]
• [Physiological interaction of vibrotactile and auditory reception]
• [Preventive effect of carotene-containing preparations in acute gastritis model]
• [Resistance of Eimeria tenella to coccidiostatic drugs]
• [Resistance of Eimeria tenella to pharmcoccide]
• [Resistance of coccidia of chickens to chemotherapeutic preparations (review)]
• [THE EFFECT OF PENICILLIN ON THE DISTRIBUTION OF SULFANILAMIDES IN THE BODY AFTER PROLONGED USE.]