SELDI-TOF plasma profiles distinguish individuals in a protein C-deficient family with thrombotic episodes occurring before age 40.

SELDI-TOF plasma profiles distinguish individuals in a protein C-deficient family with thrombotic episodes occurring before age 40. Research Abstract Details 

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SELDI-TOF plasma profiles distinguish individuals in a protein C-deficient family with thrombotic episodes occurring before age 40. Abstract Text:

We tested the hypothesis that differences in the low-molecular-weight (500-20,000 Da) proteomic profile of plasma may be detectable between members of a protein C-deficient family who have suffered thrombotic events before age 40 compared to family members without a history of venous thrombosis. Unfractionated plasma samples from members of a previously described large thrombophilic kindred with type I protein C deficiency were applied to ProteinChip weak cation exchange interaction arrays (WCX2; Ciphergen Biosystems, Fremont, CA, USA) and subjected to SELDI-TOF (surface-enhanced laser desorption/ionization time-of-flight) mass spectrometry using the Ciphergen PBSII ProteinChip System (Ciphergen Biosystems). Profiles were analyzed by a boosted decision-tree algorithm. When individuals who had presented with deep venous thrombosis (DVT) before the age of 40 (n = 21) were compared to age-matched, healthy family members (n = 50), the proteomic patterns defined by the decision-tree analysis could classify the entity of DVT before age 40 with 67% sensitivity, at a specificity of 86%. When a small group of cases with history of superficial venous thrombosis (n = 6) was added to the case group, the sensitivity was 87.5% at a specificity of 80%. These data support the hypothesis that members of the protein C deficient Vermont kindred II who suffer a thrombotic event before age 40 display significant differences in low-molecular-weight proteomics profile compared to those who remain disease-free. This is the first study to apply SELDI-TOF technology in conjunction with a bioinformatics tool to analyze low-molecular-weight proteomic patterns in patients with venous thrombosis.

SELDI-TOF plasma profiles distinguish individuals in a protein C-deficient family with thrombotic episodes occurring before age 40. Publishing Authors By Initials

For similar cardiovascular diseases: vascular diseases: embolism and thrombosis: thrombosis: venous thrombosis research abstracts see: cardiovascular diseases: vascular diseases: embolism and thrombosis: thrombosis: venous thrombosis research

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SELDI-TOF plasma profiles distinguish individuals in a protein C-deficient family with thrombotic episodes occurring before age 40. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Thrombosis and haemostasis

VOLUME: 96

Page Numbers: 725-30

Journal Abbreviation: Thromb. Haemost.

ISSN: 0340-6245

DAY: 3

MONTH: Dec

YEAR: 2006

SELDI-TOF plasma profiles distinguish individuals in a protein C-deficient family with thrombotic episodes occurring before age 40. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7608063

SELDI-TOF plasma profiles distinguish individuals in a protein C-deficient family with thrombotic episodes occurring before age 40. Keywords Mesh Terms:

KEYWORDS: Venous Thrombosis

MESH TERMS: etiology

Chemical & Substance for Abstract: SELDI-TOF plasma profiles distinguish individuals in a protein C-deficient family with thrombotic episodes occurring before age 40. Information

Substance Name: Proteins

Registry Number: 0

Grant and Affiliation Information for SELDI-TOF plasma profiles distinguish individuals in a protein C-deficient family with thrombotic episodes occurring before age 40.

AFFILIATION: Department of Pathology, University of Vermont College of Medicine, Burlington, Vermont, USA.

Country: Germany

AGENCY: United States NHLBI

GRANT: P01 HL 4703

ACRONYM: HL

MEDLINETA: Thromb Haemost

REFSOURCE: Thromb Haemost. 2006 Dec;96(6):697

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