Second generation competitive enzyme immunoassay for detection of bovine antibody to Brucella abortus.

Second generation competitive enzyme immunoassay for detection of bovine antibody to Brucella abortus. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Second generation competitive enzyme immunoassay for detection of bovine antibody to Brucella abortus. Abstract Text:

K Nielsen,P Smith,W L Yu,C Elmgren,P Nicoletti,B Perez,R Bermudez,T Renteria,K Nielsen,P Smith,W L Yu,C Elmgren,P Nicoletti,B Perez,R Bermudez,T Renteria,

A second generation competitive enzyme immunoassay (CELISA) for detection of bovine antibody to Brucella abortus was developed. This assay was different from previously developed CELISAs in that the detection reagent used was a recombinant combination of the receptor portions of protein A and protein G, labelled with horseradish peroxidase. This eliminates the need for polyclonal anti-mouse-enzyme conjugate reagents for detection thus allowing for true standardization. The assay utilized a monoclonal antibody specific for a common epitope of the O-polysaccharide (OPS) of smooth lipopolysaccharide (SLPS) derived from B. abortus S1119.3 but which did not react with protein A/G. This monoclonal antibody was used to compete with antibody in the bovine test serum. Binding of bovine antibody to the smooth lipopolysaccharide antigen was then measured directly with the protein A/G enzyme conjugate. In this case, development of colour in the reaction was indicative of the presence of bovine antibody. The performance characteristics, sensitivity, specificity and exclusion of B. abortus S19 vaccinated animals, of the assay were very similar to those of the classical CELISA.

Second generation competitive enzyme immunoassay for detection of bovine antibody to Brucella abortus. Publishing Authors By Initials

K Nielsen,P Smith,WL Yu,C Elmgren,P Nicoletti,B Perez,R Bermudez,T Renteria,K Nielsen,P Smith,WL Yu,C Elmgren,P Nicoletti,B Perez,R Bermudez,T Renteria,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Second generation competitive enzyme immunoassay for detection of bovine antibody to Brucella abortus. Journal Published:

PUBLICATION TYPE: Validation Studies

Journal: Veterinary microbiology

VOLUME: 124

Page Numbers: 173-7

Journal Abbreviation: Vet. Microbiol.

ISSN: 0378-1135

DAY: 30

MONTH: 03

YEAR: 2007

Second generation competitive enzyme immunoassay for detection of bovine antibody to Brucella abortus. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7705469

Second generation competitive enzyme immunoassay for detection of bovine antibody to Brucella abortus. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Second generation competitive enzyme immunoassay for detection of bovine antibody to Brucella abortus. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Second generation competitive enzyme immunoassay for detection of bovine antibody to Brucella abortus.

AFFILIATION: Canadian Food Inspection Agency, Ottawa Laboratory Fallowfield, 3851 Fallowfield Road, Ottawa, Ontario, Canada K2H 8P9. nielsenk@inspection.gc.ca

Country: Netherlands

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Vet Microbiol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Second generation competitive enzyme immunoassay for detection of bovine antibody to Brucella abortus Related Publications

• Second generation competitive enzyme immunoassay for detection of bovine antibody to Brucella abortus.
• Validation of a second generation competitive enzyme immunoassay (CELISA) for the diagnosis of brucellosis in various species of domestic animals.
• [Female genital mutilation--need for answers at the age of adolescence]
• [Sexual abuse of female children and adolescents--detection, examination and primary care]