Search for a dextransucrase minimal motif involved in dextran binding.

Search for a dextransucrase minimal motif involved in dextran binding. Research Abstract Details 

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Search for a dextransucrase minimal motif involved in dextran binding. Abstract Text:

Surisa Suwannarangsee,Claire Moulis,Gabrielle Potocki-Veronese,Pierre Monsan,Magali Remaud-Simeon,Warawut Chulalaksananukul,

Fourteen truncated forms of Leuconostoc mesenteroides NRRL B512-F dextransucrase, involving N-, C- or N- plus C-terminal domain truncations were tested for their ability to bind dextrans. The shortest fragment (14kDa molecular weight) that still exhibited a strong interaction with dextran was localized between amino acids N1397 and A1527 of the C-terminal domain (GBD-7) and consists of six YG repeats. With a dissociation constant K(d) of 2.8x10(-9)M, this motif shows a very high affinity for isomaltohexaose and longer dextrans, supporting the proposed role of GBD in polymer formation. The potential application of GBD-7 as an affinity tag onto cheap resins like Sephacryl S300HR for rapid purification was evaluated and is discussed.

Search for a dextransucrase minimal motif involved in dextran binding. Publishing Authors By Initials

S Suwannarangsee,C Moulis,G Potocki-Veronese,P Monsan,M Remaud-Simeon,W Chulalaksananukul,

For similar proteins: thioredoxins research abstracts see: proteins: thioredoxins research

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Search for a dextransucrase minimal motif involved in dextran binding. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: FEBS letters

VOLUME: 581

Page Numbers: 4675-80

Journal Abbreviation: FEBS Lett.

ISSN: 0014-5793

DAY: 4

MONTH: 09

YEAR: 2007

Search for a dextransucrase minimal motif involved in dextran binding. Information

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LANGUAGE: eng

NlmUniqueID: 155157

Search for a dextransucrase minimal motif involved in dextran binding. Keywords Mesh Terms:

KEYWORDS: Thioredoxins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Search for a dextransucrase minimal motif involved in dextran binding. Information

Substance Name: dextransucrase

Registry Number: EC 2.4.1.5

Grant and Affiliation Information for Search for a dextransucrase minimal motif involved in dextran binding.

AFFILIATION: Biological Science Program, Faculty of Science, Chulalongkorn University, 254 Phyathai Road, Patumwan, Bangkok, Thailand.

Country: Netherlands

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MEDLINETA: FEBS Lett

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