Sea urchin protease specific to the SPKK motif in histone.

Sea urchin protease specific to the SPKK motif in histone. Research Abstract Details 

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Sea urchin protease specific to the SPKK motif in histone. Abstract Text:

M Suzuki,M Sugiura,S Ebashi,

A protease activity specific to spermatogenous histones was found in the egg extract of sea urchin. The enzyme responsible for this activity, named SPKK protease because of its substrate specificity, was purified as a monomeric 28 kDa protein. SPKK protease activity is inhibited by leupeptin and is specific to the repeat of sequences like Ser-Pro-Lys-Lys (the SPKK motif) [Suzuki, M. (1989), EMBO J. 8, 797-804]. The DNA-binding sites of sea urchin spermatogenous histones H1 and H2B, which protect the linker DNA of chromatin, are made up of sequences rich in the SPKK motif. SPKK protease may contribute not only to the unpacking of sperm chromatin but also to transcription activation of the male origin gene at fertilisation. SPKK protease resembles another protease activity on nucleolin [Burger et al. (1982) Eur. J. Biochem. 128 475-480] in its characteristics.

Sea urchin protease specific to the SPKK motif in histone. Publishing Authors By Initials

M Suzuki,M Sugiura,S Ebashi,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: substrate specificity research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: substrate specificity research

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Sea urchin protease specific to the SPKK motif in histone. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 108

Page Numbers: 347-55

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 1990

Sea urchin protease specific to the SPKK motif in histone. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Sea urchin protease specific to the SPKK motif in histone. Keywords Mesh Terms:

KEYWORDS: Substrate Specificity

MESH TERMS: drug effects

Chemical & Substance for Abstract: Sea urchin protease specific to the SPKK motif in histone. Information

Substance Name: SPKK protease

Registry Number: EC 3.4.99.-

Grant and Affiliation Information for Sea urchin protease specific to the SPKK motif in histone.

AFFILIATION: National Institute for Physiological Sciences, Aichi.

Country: JAPAN

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MEDLINETA: J Biochem

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