SDS-polyacrylamide gel electrophoresis of buffalo bulls seminal plasma proteins and their relation with semen freezability.

SDS-polyacrylamide gel electrophoresis of buffalo bulls seminal plasma proteins and their relation with semen freezability. Research Abstract Details 

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SDS-polyacrylamide gel electrophoresis of buffalo bulls seminal plasma proteins and their relation with semen freezability. Abstract Text:

R Asadpour,S M Alavi-Shoushtari,S Asri Rezaii,M H Kh Ansari,R Asadpour,S M Alavi-Shoushtari,S Asri Rezaii,M H Kh Ansari,

The objective of this study was to evaluate the protein profiles of seminal plasma in buffalo bulls and to examine their correlation with semen characteristics. Semen of 10 buffalo bulls were collected by a bovine artificial vagina. Semen characteristics (motility, morphology, viability and concentration) were recorded. A part of the semen sample (1 ml) was diluted by tris-egg yolk-glycerol extender, packed in French straws and was frozen in liquid nitrogen. The straws were later thawed and semen characteristics were compared with those of the fresh semen. Seminal plasma was harvested by centrifugation; treated with cold ethanol and then, underwent SDS-polyacrylamide gel electrophoresis (PAGE). Twenty five protein bands were identified on the gel, of which those of <35.5 kDa were prominent (72% of the bands). Of these protein fractions, 24.5 kDa was significantly correlated with sperm progressive motility in fresh and viability in frozen-thawed semen while 45 kDa bands were correlated with abnormal morphology in frozen-thawed semen; 55 kDa protein fractions were correlated with sperm viability of fresh semen. Progressive motility, viability and abnormal sperm morphology of frozen-thawed semen were highly correlated with these parameters in the fresh semen. In conclusion, seminal plasma protein fractions in buffalo bulls are similar to those reported in other animal species and have some correlations with semen characteristics before and after freezing.

SDS-polyacrylamide gel electrophoresis of buffalo bulls seminal plasma proteins and their relation with semen freezability. Publishing Authors By Initials

R Asadpour,SM Alavi-Shoushtari,SA Rezaii,MH Ansari,R Asadpour,SM Alavi-Shoushtari,SA Rezaii,MH Ansari,

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SDS-polyacrylamide gel electrophoresis of buffalo bulls seminal plasma proteins and their relation with semen freezability. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Animal reproduction science

VOLUME: 102

Page Numbers: 308-13

Journal Abbreviation: Anim. Reprod. Sci.

ISSN: 0378-4320

DAY: 6

MONTH: 03

YEAR: 2007

SDS-polyacrylamide gel electrophoresis of buffalo bulls seminal plasma proteins and their relation with semen freezability. Information

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LANGUAGE: eng

NlmUniqueID: 7807205

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Grant and Affiliation Information for SDS-polyacrylamide gel electrophoresis of buffalo bulls seminal plasma proteins and their relation with semen freezability.

AFFILIATION: Department of Veterinary Clinical Science, Division of Theriogenology, School of Veterinary Medicine, University of Urmia, Urmia 57153, Iran.

Country: Netherlands

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MEDLINETA: Anim Reprod Sci

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