Screening the sequence selectivity of DNA-binding molecules using a gold nanoparticle-based colorimetric approach.

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Screening the sequence selectivity of DNA-binding molecules using a gold nanoparticle-based colorimetric approach. Abstract Text:

Sarah J Hurst,Min Su Han,Abigail K R Lytton-Jean,Chad A Mirkin,Sarah J Hurst,Min Su Han,Abigail K R Lytton-Jean,Chad A Mirkin,

We have developed a novel competition assay that uses a gold nanoparticle (Au NP)-based, high-throughput colorimetric approach to screen the sequence selectivity of DNA-binding molecules. This assay hinges on the observation that the melting behavior of DNA-functionalized Au NP aggregates is sensitive to the concentration of the DNA-binding molecule in solution. When short, oligomeric hairpin DNA sequences were added to a reaction solution consisting of DNA-functionalized Au NP aggregates and DNA-binding molecules, these molecules may either bind to the Au NP aggregate interconnects or the hairpin stems based on their relative affinity for each. This relative affinity can be measured as a change in the melting temperature (Tm) of the DNA-modified Au NP aggregates in solution. As a proof of concept, we evaluated the selectivity of 4',6-diamidino-2-phenylindone (an AT-specific binder), ethidium bromide (a nonspecific binder), and chromomycin A (a GC-specific binder) for six sequences of hairpin DNA having different numbers of AT pairs in a five-base pair variable stem region. Our assay accurately and easily confirmed the known trends in selectivity for the DNA binders in question without the use of complicated instrumentation. This novel assay will be useful in assessing large libraries of potential drug candidates that work by binding DNA to form a drug/DNA complex.

Screening the sequence selectivity of DNA-binding molecules using a gold nanoparticle-based colorimetric approach. Publishing Authors By Initials

SJ Hurst,MS Han,AK Lytton-Jean,CA Mirkin,SJ Hurst,MS Han,AK Lytton-Jean,CA Mirkin,

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Screening the sequence selectivity of DNA-binding molecules using a gold nanoparticle-based colorimetric approach. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Analytical chemistry

VOLUME: 79

Page Numbers: 7201-5

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 15

MONTH: 08

YEAR: 2007

Screening the sequence selectivity of DNA-binding molecules using a gold nanoparticle-based colorimetric approach. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Screening the sequence selectivity of DNA-binding molecules using a gold nanoparticle-based colorimetric approach.

AFFILIATION: Department of Chemistry and International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208-3113, USA.

Country: United States

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MEDLINETA: Anal Chem

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