[Screening of TNF-alpha mimotopes from c7c phage display peptide library]

[Screening of TNF-alpha mimotopes from c7c phage display peptide library] Research Abstract Details 

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[Screening of TNF-alpha mimotopes from c7c phage display peptide library] Abstract Text:

Hai-Bo Luo,Bei-Yi Liu,Yan-Jun Liu,Ping Zhu,Ning Fu,

AIM:To acquire oligo-peptides mimicking TNF-alpha epitopes. METHODS: The TNF-alpha mimotopes were screened from c7c phage display peptide library by using neutralizing mAb J1D9 against TNF-alpha, and positive clones were identified by sandwich ELISA. RESULTS: 9 out of 20 phage clones were identified as positive clones which can bind to mAb J1D9. The deduced amino acid sequence analysis showed three different sequences: c-RRPAQSG-c, c-NKHNRKI-c and c-RGMSRKI-c. CONCLUSION: All of the three peptides obtained can mimic the epitopes of TNF-alpha and bind to mAb J1D9.

[Screening of TNF-alpha mimotopes from c7c phage display peptide library] Publishing Authors By Initials

HB Luo,BY Liu,YJ Liu,P Zhu,N Fu,

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[Screening of TNF-alpha mimotopes from c7c phage display peptide library] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese jour

VOLUME: 19

Page Numbers: 322-5

Journal Abbreviation: Xi Bao Yu Fen Zi Mian Yi Xue Z

ISSN: 1007-8738

DAY: 27

MONTH: Jul

YEAR: 2003

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LANGUAGE: chi

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AFFILIATION: Department of Immunology, First Military Medical University, Guangzhou 510515, China.

Country: China

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MEDLINETA: Xi Bao Yu Fen Zi Mian Yi Xue Z

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