Screening for compounds that induce apoptosis of cancer cells grown as multicellular spheroids.

Screening for compounds that induce apoptosis of cancer cells grown as multicellular spheroids. Research Abstract Details 

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Screening for compounds that induce apoptosis of cancer cells grown as multicellular spheroids. Abstract Text:

Richard Herrmann,Walid Fayad,Stephan Schwarz,Maria Berndtsson,Stig Linder,Richard Herrmann,Walid Fayad,Stephan Schwarz,Maria Berndtsson,Stig Linder,

Screening and initial characterization of anticancer drugs are typically performed using monolayer cultures of tumor cells. It is well established that such monolayer cultures do not represent the characteristics of 3-dimensional solid tumors. The multicellular tumor spheroid model is of intermediate complexity between in vivo tumors and in vitro monolayer cultures and would be more suitable for drug screening. The authors describe a procedure in which multicellular spheroids are used to screen for compounds that induce tumor cell apoptosis. Multicellular spheroids were generated in 96-well plates, and apoptosis was determined using the M30-Apoptosense( (R)) enzyme-linked immunosorbent assay method. A Z' factor of approximately 0.5 was observed for HCT116 colon carcinoma spheroids using staurosporine to induce apoptosis. This procedure is attractive for secondary screening of hits from larger cell-based screens.

Screening for compounds that induce apoptosis of cancer cells grown as multicellular spheroids. Publishing Authors By Initials

R Herrmann,W Fayad,S Schwarz,M Berndtsson,S Linder,R Herrmann,W Fayad,S Schwarz,M Berndtsson,S Linder,

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Screening for compounds that induce apoptosis of cancer cells grown as multicellular spheroids. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biomolecular screening : the official j

VOLUME: 13

Page Numbers: 1-8

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ISSN: 1087-0571

DAY: 26

MONTH: 11

YEAR: 2007

Screening for compounds that induce apoptosis of cancer cells grown as multicellular spheroids. Information

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LANGUAGE: eng

NlmUniqueID: 9612112

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Grant and Affiliation Information for Screening for compounds that induce apoptosis of cancer cells grown as multicellular spheroids.

AFFILIATION: Cancer Center Karolinska, Department of Oncology and Pathology, Karolinska Institute and Hospital, Stockholm, Sweden.

Country: United States

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MEDLINETA: J Biomol Screen

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