[Screening and identification of egg mimotope of Schistosoma japonicum]

[Screening and identification of egg mimotope of Schistosoma japonicum] Research Abstract Details 

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[Screening and identification of egg mimotope of Schistosoma japonicum] Abstract Text:

Min Qian,Yan-jun Bai,Ping Zhang,Zi-zhu Yan,Zi-rong Wu,

OBJECTIVE: To explore egg mimotope of Schistosoma japonicum that can be used in the development of diagnostic reagents for schistosomiasis. METHODS: By performing three rounds of biopanning in the affinity selection before picking out single clones for identification, target specific phages were effectively enriched from a random 15-peptides phage library with an immobilized mAb 6B12 which is specific to egg antigen of S. japonicum as a bait. By using ELISA, competitive ELISA with natural egg antigen as competitor, and Western blotting, 13 phage clones with high affinity and specificity to 6B12 were obtained and amplified from 400 single clones. RESULTS: DNA sequencing revealed that all the 13 selected single clones were identical in displaying gene sequence. Serum samples were tested by ELISA for the presence of IgG antibodies to the phages with mimic epitope, and showed that there was a significant difference between healthy volunteers and schistosomiasis patients. CONCLUSION: It is possible that the phage display peptide antigen, egg mimotope of S. japonicum may be a replacement of natural egg antigen for the diagnosis of schistosomiasis.

[Screening and identification of egg mimotope of Schistosoma japonicum] Publishing Authors By Initials

M Qian,YJ Bai,P Zhang,ZZ Yan,ZR Wu,

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[Screening and identification of egg mimotope of Schistosoma japonicum] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zhongguo ji sheng chong xue yu ji sheng chong bing

VOLUME: 20

Page Numbers: 1-5

Journal Abbreviation: Zhongguo Ji Sheng Chong Xue Yu

ISSN: 1000-7423

DAY: 5

MONTH: 02

YEAR: 2002

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LANGUAGE: chi

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AFFILIATION: School of Life Science, East China Normal University, Shanghai 200062.

Country: China

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MEDLINETA: Zhongguo Ji Sheng Chong Xue Yu

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