Schizosaccharomyces pombe switches mating type by the synthesis-dependent strand-annealing mechanism.

Schizosaccharomyces pombe switches mating type by the synthesis-dependent strand-annealing mechanism. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Schizosaccharomyces pombe switches mating type by the synthesis-dependent strand-annealing mechanism. Abstract Text:

Tomoko Yamada-Inagawa,Amar J S Klar,Jacob Z Dalgaard,Tomoko Yamada-Inagawa,Amar J S Klar,Jacob Z Dalgaard,

Schizosaccharomyces pombe cells can switch between two mating types, plus (P) and minus (M). The change in cell type occurs due to a replication-coupled recombination event that transfers genetic information from one of the silent-donor loci, mat2P or mat3M, into the expressed mating-type determining mat1 locus. The mat1 locus can as a consequence contain DNA encoding either P or M information. A molecular mechanism, known as synthesis-dependent strand annealing, has been proposed for the underlying recombination event. A key feature of this model is that only one DNA strand of the donor locus provides the information that is copied into the mat1. Here we test the model by constructing strains that switch using two different mutant P cassettes introduced at the donor loci, mat2 and mat3. We show that in such strains wild-type P-cassette DNA is efficiently generated at mat1 through heteroduplex DNA formation and repair. The present data provide an in vivo genetic test of the proposed molecular recombination mechanism.

Schizosaccharomyces pombe switches mating type by the synthesis-dependent strand-annealing mechanism. Publishing Authors By Initials

T Yamada-Inagawa,AJ Klar,JZ Dalgaard,T Yamada-Inagawa,AJ Klar,JZ Dalgaard,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Schizosaccharomyces pombe switches mating type by the synthesis-dependent strand-annealing mechanism. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Genetics

VOLUME: 177

Page Numbers: 255-65

Journal Abbreviation: Genetics

ISSN: 0016-6731

DAY: 29

MONTH: 07

YEAR: 2007

Schizosaccharomyces pombe switches mating type by the synthesis-dependent strand-annealing mechanism. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 374636

Schizosaccharomyces pombe switches mating type by the synthesis-dependent strand-annealing mechanism. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Schizosaccharomyces pombe switches mating type by the synthesis-dependent strand-annealing mechanism. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Schizosaccharomyces pombe switches mating type by the synthesis-dependent strand-annealing mechanism.

AFFILIATION: Marie Curie Research Institute, Surry RH8 0TL, United Kingdom.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Genetics

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Schizosaccharomyces pombe switches mating type by the synthesis-dependent strand-annealing mechanism Related Publications

• Aberrant thyroid tissue.
• Acute lesions of the brain and recent gastro-duodenal ulcers or gastromalacia acida.
• Fatal dextropropoxyphene poisonings in Jutland, Denmark.
• Intracranial infections causing esophagomalacia and gastromalacia: a postmortem study of eleven cases. V. Ulcer and brain.
• Modeling and predicting the growth of lactic acid bacteria in lightly preserved seafood and their inhibiting effect on Listeria monocytogenes.
• New simple carbon monoxide detector.
• Phosphatase in dogs following bile obstruction and removal of the small intestine.
• Pial aneurysms in a diabetic patient.
• Post mortem findings in carbon monoxide deaths.
• Rtf1-mediated eukaryotic site-specific replication termination.
• Schizosaccharomyces pombe switches mating type by the synthesis-dependent strand-annealing mechanism.
• Serum and bile phosphatase in biliary fistula dogs.
• Serum phosphatase after hepatectomy in dogs.
• Serum phosphatase after nephrectomy and bile obstruction in dogs.
• Tracer phosphatase determination in tissue sections.
• [A case of dicumarol poisoning.]
• [Dicumarol intoxication, its prevention and therapy.]
• [Family epidemic of ornithosis with interhuman transmission.]
• [Not Available.]
• [Not Available.]
• [Would a crash helmet have been life-saving? An analysis of 188 fatal motor scooter accidents (author's transl)]