Scaling down: a PCR-based method to efficiently screen for desired knockouts in a high density Mycobacterium tuberculosis picked mutant library.

Scaling down: a PCR-based method to efficiently screen for desired knockouts in a high density Mycobacterium tuberculosis picked mutant library. Research Abstract Details 

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Scaling down: a PCR-based method to efficiently screen for desired knockouts in a high density Mycobacterium tuberculosis picked mutant library. Abstract Text:

James M Lane,Eric J Rubin,

Transposon mutagenesis produces random mycobacterial mutants at high frequency. Because they are random, however, it is difficult to isolate mutations in particular target genes. Here we describe the use of an arrayed library of Mycobacterium tuberculosis together with a PCR screening strategy to rapidly identify strains with defined insertion mutations. This method is useful for many genetic applications.

Scaling down: a PCR-based method to efficiently screen for desired knockouts in a high density Mycobacterium tuberculosis picked mutant library. Publishing Authors By Initials

JM Lane,EJ Rubin,

For similar investigative techniques: genetic techniques: nucleic acid amplification techniques: polymerase chain reaction research abstracts see: investigative techniques: genetic techniques: nucleic acid amplification techniques: polymerase chain reaction research

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Scaling down: a PCR-based method to efficiently screen for desired knockouts in a high density Mycobacterium tuberculosis picked mutant library. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Tuberculosis (Edinburgh, Scotland)

VOLUME: 86

Page Numbers: 310-3

Journal Abbreviation:

ISSN: 1472-9792

DAY: 9

MONTH: 03

YEAR: 2006

Scaling down: a PCR-based method to efficiently screen for desired knockouts in a high density Mycobacterium tuberculosis picked mutant library. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100971555

Scaling down: a PCR-based method to efficiently screen for desired knockouts in a high density Mycobacterium tuberculosis picked mutant library. Keywords Mesh Terms:

KEYWORDS: Polymerase Chain Reaction

MESH TERMS: methods

Chemical & Substance for Abstract: Scaling down: a PCR-based method to efficiently screen for desired knockouts in a high density Mycobacterium tuberculosis picked mutant library. Information

Substance Name: DNA, Bacterial

Registry Number: 0

Grant and Affiliation Information for Scaling down: a PCR-based method to efficiently screen for desired knockouts in a high density Mycobacterium tuberculosis picked mutant library.

AFFILIATION: Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA.

Country: Scotland

AGENCY: United States NIAID

GRANT: AI51929

ACRONYM: AI

MEDLINETA: Tuberculosis (Edinb)

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