Scaffold proteins and the regeneration of visual pigments.

Scaffold proteins and the regeneration of visual pigments. Research Abstract Details 

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Scaffold proteins and the regeneration of visual pigments. Abstract Text:

Maria Nawrot,Tianyun Liu,Gregory G Garwin,John W Crabb,John C Saari,

CRALBP, cellular retinaldehyde-binding protein, is a retinoid-binding protein necessary for efficient regeneration of rod and cone visual pigments. The C terminus of CRALBP binds to the PDZ domains of EBP50/NHERF-1, which in turn bind to ezrin and actin, proteins localized to the apical processes of the retinal pigment epithelium. In this study, we examined structural features associated with the interaction of the two proteins. The C-terminal amino-acid sequence of 11 orthologous CRALBPs is either ENTAL, ENTAF or EDTAL. Peptides ending in each of these sequences inhibited the interaction of CRALBP and EBP50/NHERF-1 with the use of an overlay assay. Molecular modeling showed that both NTAL and NTAF formed similar networks of H bonds with PDZ1 of EBP50/ NHERF-1, and the side chains of both C-terminal Leu and Phe fit into the peptide-binding groove of PDZ1x CRALBP.11-cis-retinal and EBP50/NHERF-1 migrated as single components when analyzed individually by gel filtration and as a complex when mixed together before gel filtration. Complex formation was abolished by preincubation of EBP50/NHERF-1 with peptide EVENTAL. The ligand absorption spectrum of the complex was identical with that of CRALBP x 11-cis-retinal, demonstrating that complex formation did not perturb the ligand-binding domain of CRALBP.

Scaffold proteins and the regeneration of visual pigments. Publishing Authors By Initials

M Nawrot,T Liu,GG Garwin,JW Crabb,JC Saari,

For similar organic chemicals: aldehydes: retinaldehyde research abstracts see: organic chemicals: aldehydes: retinaldehyde research

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Scaffold proteins and the regeneration of visual pigments. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Photochemistry and photobiology

VOLUME: 82

Page Numbers: 1482-8

Journal Abbreviation: Photochem. Photobiol.

ISSN: 0031-8655

DAY: 3

MONTH: 12

YEAR: 2007

Scaffold proteins and the regeneration of visual pigments. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376425

Scaffold proteins and the regeneration of visual pigments. Keywords Mesh Terms:

KEYWORDS: Retinaldehyde

MESH TERMS: metabolism

Chemical & Substance for Abstract: Scaffold proteins and the regeneration of visual pigments. Information

Substance Name: Retinaldehyde

Registry Number: 116-31-4

Grant and Affiliation Information for Scaffold proteins and the regeneration of visual pigments.

AFFILIATION: Department of Ophthalmology, University of Washington, Seattle, WA, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM63020

ACRONYM: GM

MEDLINETA: Photochem Photobiol

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