Saturation of the Secretory Pathway by Overexpression of a Hookworm (Necator americanus) Protein (Na-ASP1).

Saturation of the Secretory Pathway by Overexpression of a Hookworm (Necator americanus) Protein (Na-ASP1). Research Abstract Details 

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Saturation of the Secretory Pathway by Overexpression of a Hookworm (Necator americanus) Protein (Na-ASP1). Abstract Text:

Mehmet Inan,Sarah A Fanders,Wenhui Zhang,Peter J Hotez,Bin Zhan,Michael M Meagher,Mehmet Inan,Sarah A Fanders,Wenhui Zhang,Peter J Hotez,Bin Zhan,Michael M Meagher,Mehmet Inan,Sarah A Fanders,Wenhui Zhang,Peter J Hotez,Bin Zhan,Michael M Meagher,

Human hookworm infection is one of the most significant parasitic infections, and a leading global cause of anemia and malnutrition of adults and children in rural areas of the tropics and subtropics. Necator americanus secretory protein (Na-ASP1), which is a potential vaccine candidate against hookworm infections, has been expressed in Pichia pastoris. Na-ASP1 protein was expressed extracellulary by employing the leader sequence of the alpha-mating factor of Saccharomyces cerevisiae. Most of the protein produced by single copy clones was secreted outside the cell. The Na-ASP1 steady state mRNA levels of the clones were correlated to their Na-ASP1 gene copy number. However, increasing gene copy number of Na-ASP1 protein in P. pastoris saturated secretory capacity and therefore, decreased the amount of secreted protein in clones harboring multiple copies of Na-ASP1 gene.

Saturation of the Secretory Pathway by Overexpression of a Hookworm (Necator americanus) Protein (Na-ASP1). Publishing Authors By Initials

M Inan,SA Fanders,W Zhang,PJ Hotez,B Zhan,MM Meagher,M Inan,SA Fanders,W Zhang,PJ Hotez,B Zhan,MM Meagher,M Inan,SA Fanders,W Zhang,PJ Hotez,B Zhan,MM Meagher,

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Saturation of the Secretory Pathway by Overexpression of a Hookworm (Necator americanus) Protein (Na-ASP1). Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Methods in molecular biology (Clifton, N.J.)

VOLUME: 389

Page Numbers: 65-76

Journal Abbreviation: Methods Mol. Biol.

ISSN: 1064-3745

DAY: 22

MONTH: 10

YEAR: 2007

Saturation of the Secretory Pathway by Overexpression of a Hookworm (Necator americanus) Protein (Na-ASP1). Information

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LANGUAGE: eng

NlmUniqueID: 9214969

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