Salt effects on protein-DNA interactions. The lambda cI repressor and EcoRI endonuclease.

Salt effects on protein-DNA interactions. The lambda cI repressor and EcoRI endonuclease. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Salt effects on protein-DNA interactions. The lambda cI repressor and EcoRI endonuclease. Abstract Text:

In this paper, finite-difference solutions to the nonlinear Poisson-Boltzmann (NLPB) equation are used to calculate the salt dependent contribution to the electrostatic DNA binding free energy for both the lambda cI repressor and the EcoRI endonuclease. For the protein-DNA systems studied, the NLPB method describes nonspecific univalent salt dependent effects on the binding free energy which are in excellent agreement with experimental results. In these systems, the contribution of the ion atmosphere to the binding free energy substantially destabilizes the protein-DNA complexes. The magnitude of this effect involves a macromolecular structure dependent redistribution of both cations and anions around the protein and the DNA which is dominated by long range electrostatic interactions. We find that the free energy associated with global ion redistribution upon binding is more important than changes associated with local protein-DNA interactions (ion-pairs) in determining salt effects. The NLPB model reveals how long range salt effects can play a significant role in the relative stability of protein-DNA complexes with different structures.

Salt effects on protein-DNA interactions. The lambda cI repressor and EcoRI endonuclease. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Salt effects on protein-DNA interactions. The lambda cI repressor and EcoRI endonuclease. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of molecular biology

VOLUME: 238

Page Numbers: 264-80

Journal Abbreviation: J. Mol. Biol.

ISSN: 0022-2836

DAY: 29

MONTH: Apr

YEAR: 1994

Salt effects on protein-DNA interactions. The lambda cI repressor and EcoRI endonuclease. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985088

Salt effects on protein-DNA interactions. The lambda cI repressor and EcoRI endonuclease. Keywords Mesh Terms:

KEYWORDS: Viral Regulatory and Accessory Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Salt effects on protein-DNA interactions. The lambda cI repressor and EcoRI endonuclease. Information

Substance Name: Deoxyribonuclease EcoRI

Registry Number: EC 3.1.21.-

Grant and Affiliation Information for Salt effects on protein-DNA interactions. The lambda cI repressor and EcoRI endonuclease.

AFFILIATION: Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032.

Country: ENGLAND

AGENCY: United States NIGMS

GRANT: GM-41371

ACRONYM: GM

MEDLINETA: J Mol Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Salt effects on protein-DNA interactions The lambda cI repressor and EcoRI endonuclease Related Publications

• A BIOCHEMICAL MECHANISM BY WHICH ADRENERGIC MEDIATORS MODIFY CARDIAC CONTRACTION.
• Acne arising in an epidermal nevus.
• Beta-thromboglobulin and platelet factor 4 levels in post-myocardial infarction patients with major depression.
• Cell-to-cell contact and extracellular matrix.
• Comparative study of generalized born models: Born radii and peptide folding.
• Diaper rash in a young infant.
• Electrocardiograms of more than routine interest. The electrocardiogram in a patient with mitral atresia and single functional ventricle.
• Etretinate therapy for generalized pustular psoriasis in children.
• Experimental fusion of the naturally cleft, embryonic chick palate.
• Fluvoxamine versus fluoxetine in major depressive episode: a double-blind randomised comparison.
• Identification of a chloride ion binding site in Na+/Cl -dependent transporters.
• Intention tremor of the head in patients with essential tremor.
• Labelling alcoholic drinks: percentage proof, original gravity, percentage alcohol or standard drinks?
• Laboratory biomarkers in Alzheimer's disease.
• Local quality assessment in homology models using statistical potentials and support vector machines.
• Loop modeling: Sampling, filtering, and scoring.
• Nuclear localization and cytosolic overexpression of LASP-1 correlates with tumor size and nodal-positivity of human breast carcinoma.
• Nuclear spin resonance of (129)Xe doped with O(2).
• Prediction of side-chain conformations on protein surfaces.
• Protein structure space is much more than the sum of its folds.
• Salt effects on polyelectrolyte-ligand binding: comparison of Poisson-Boltzmann, and limiting law/counterion binding models.
• Salt effects on protein-DNA interactions. The lambda cI repressor and EcoRI endonuclease.
• Secondary ion quadrupole mass spectrometer for depth profiling-design and performance evaluation.
• Sequence and structural determinants of strand swapping in cadherin domains: do all cadherins bind through the same adhesive interface?
• Structural refinement of protein segments containing secondary structure elements: Local sampling, knowledge-based potentials, and clustering.
• Treatment of post-myocardial infarction depressive disorder.
• [Chest pain due to a panic disorder: the significance of a multidisciplinary approach]
• [In Process Citation]
• [Mortality in pregnant and newly delivered women due to psychiatric disorders]
• [The placing of an unborn child under formal supervision where there is doubt about the safety of the home situation]