Salmonella enterica serovar Typhimurium regulates intercellular junction proteins and facilitates transepithelial neutrophil and bacterial passage.

Salmonella enterica serovar Typhimurium regulates intercellular junction proteins and facilitates transepithelial neutrophil and bacterial passage. Research Abstract Details 

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Salmonella enterica serovar Typhimurium regulates intercellular junction proteins and facilitates transepithelial neutrophil and bacterial passage. Abstract Text:

Henrik ,Takanori Sakaguchi,Bryan P Hurley,Benjamin J Kase,Hans-Christian Reinecker,Beth A McCormick,Henrik ,Takanori Sakaguchi,Bryan P Hurley,Benjamin A Kase,Benjamin J Kase,Hans-Christian Reinecker,Beth A McCormick,

The establishment of tight junctions (TJ) between columnar epithelial cells defines the functional barrier, which enteroinvasive pathogens have to overcome. Salmonella enterica serovar Typhimurium (S. typhimurium) directly invades intestinal epithelial cells but it is not well understood how the pathogen is able to overcome the intestinal barrier and gains access to the circulation. Therefore, we sought to determine whether infection with S. typhimurium could regulate the molecular composition of the TJ and, if so, whether these modifications would influence bacterial translocation and polymorphonuclear leukocyte (PMN) movement across model intestinal epithelium. We found that infection of a model intestinal epithelium with S. typhimurium over 2 h resulted in an approximately 80% loss of transepithelial electrical resistance. Western blot analysis of epithelial cell lysates demonstrated that S. typhimurium regulated the distribution of the TJ complex proteins claudin-1, zonula occludens (ZO)-2, and E-cadherin in Triton X-100-soluble and insoluble fractions. In addition, S. typhimurium was specifically able to dephosphorylate occludin and degrade ZO-1. This TJ alteration in the epithelial monolayer resulted in 10-fold increase in bacterial translocation and a 75% increase in N-formylmethionin-leucyl-phenyalanine-induced PMN transepithelial migration. Our data demonstrate that infection with S. typhimurium is associated with the rapid targeting of the tight junctional complex and loss of barrier function. This results in enhanced bacterial translocation and initiation of PMN migration across the intestinal barrier. Therefore, the ability to regulate the molecular composition of TJs facilitates the pathogenicity of S. typhimurium by aiding its uptake and distribution within the host.

Salmonella enterica serovar Typhimurium regulates intercellular junction proteins and facilitates transepithelial neutrophil and bacterial passage. Publishing Authors By Initials

H ,T Sakaguchi,BP Hurley,BJ Kase,HC Reinecker,BA McCormick,H ,T Sakaguchi,BP Hurley,BA Kase,BJ Kase,HC Reinecker,BA McCormick,

For similar cells: cellular structures: cell membrane: cell membrane structures: intercellular junctions: tight junctions research abstracts see: cells: cellular structures: cell membrane: cell membrane structures: intercellular junctions: tight junctions research

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Salmonella enterica serovar Typhimurium regulates intercellular junction proteins and facilitates transepithelial neutrophil and bacterial passage. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: American journal of physiology. Gastrointestinal a

VOLUME: 293

Page Numbers: G178-87

Journal Abbreviation: Am. J. Physiol. Gastrointest.

ISSN: 0193-1857

DAY: 3

MONTH: Jul

YEAR: 2007

Salmonella enterica serovar Typhimurium regulates intercellular junction proteins and facilitates transepithelial neutrophil and bacterial passage. Information

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LANGUAGE: eng

NlmUniqueID: 100901227

Salmonella enterica serovar Typhimurium regulates intercellular junction proteins and facilitates transepithelial neutrophil and bacterial passage. Keywords Mesh Terms:

KEYWORDS: Tight Junctions

MESH TERMS: metabolism

Chemical & Substance for Abstract: Salmonella enterica serovar Typhimurium regulates intercellular junction proteins and facilitates transepithelial neutrophil and bacterial passage. Information

Substance Name: zonula occludens-1 protein

Registry Number: 0

Grant and Affiliation Information for Salmonella enterica serovar Typhimurium regulates intercellular junction proteins and facilitates transepithelial neutrophil and bacterial passage.

AFFILIATION: Mucosal Immunology Laboratories, Massachusetts General Hospital, 114 16th Street, Charlestown, MA 02129, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: DK 56754

ACRONYM: DK

MEDLINETA: Am J Physiol Gastrointest Live

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