Salivary mucin MG1 is comprised almost entirely of different glycosylated forms of the MUC5B gene product.

Salivary mucin MG1 is comprised almost entirely of different glycosylated forms of the MUC5B gene product. Research Abstract Details 

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Salivary mucin MG1 is comprised almost entirely of different glycosylated forms of the MUC5B gene product. Abstract Text:

D J Thornton,N Khan,R Mehrotra,M Howard,E Veerman,N H Packer,J K Sheehan,

The MG1 population of mucins was isolated from human whole salivas by gel chromatography followed by isopycnic density gradient centrifugation. The reduced and alkylated MG1 mucins, separated by anion exchange chromatography, were of similar size (radius of gyration 55-64 nm) and molecular weight (2.5-2.9 x 10(6) Da). Two differently-charged populations of MG1 subunits were observed which showed different reactivity with monoclonal antibodies to glycan epitopes. Monosaccharide and amino acid compositional analyses indicated that the MG1 subunits had similar glycan structures on the same polypeptide. An antiserum recognizing the MUC5B mucin was reactive across the entire distribution, whereas antisera raised against the MUC2 and MUC5AC mucins showed no reactivity. Western blots of agarose gel electrophoresis of fractions across the anion exchange distribution indicated that the polypeptide underlying the mucins was the product of the MUC5B gene. Amino acid analysis and peptide mapping performed on the fragments produced by trypsin digestion of the two MG1 populations yielded data similar to that obtained for MUC5B mucin subunits prepared from respiratory mucus (Thornton et al., 1997) and confirmed that the MUC5B gene product was the predominant mucin polypeptide present. Isolation of the MG1 mucins from the secretions of the individual salivary glands (palatal, sublingual, and submandibular) indicate that the palatal gland is the source of the highly charged population of the MUC5B mucin.

Salivary mucin MG1 is comprised almost entirely of different glycosylated forms of the MUC5B gene product. Publishing Authors By Initials

DJ Thornton,N Khan,R Mehrotra,M Howard,E Veerman,NH Packer,JK Sheehan,

For similar salivary glands: submandibular gland research abstracts see: salivary glands: submandibular gland research

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Salivary mucin MG1 is comprised almost entirely of different glycosylated forms of the MUC5B gene product. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Glycobiology

VOLUME: 9

Page Numbers: 293-302

Journal Abbreviation: Glycobiology

ISSN: 0959-6658

DAY: 30

MONTH: Mar

YEAR: 1999

Salivary mucin MG1 is comprised almost entirely of different glycosylated forms of the MUC5B gene product. Information

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LANGUAGE: eng

NlmUniqueID: 9104124

Salivary mucin MG1 is comprised almost entirely of different glycosylated forms of the MUC5B gene product. Keywords Mesh Terms:

KEYWORDS: Submandibular Gland

MESH TERMS: secretion

Chemical & Substance for Abstract: Salivary mucin MG1 is comprised almost entirely of different glycosylated forms of the MUC5B gene product. Information

Substance Name: mucin 5AC

Registry Number: 0

Grant and Affiliation Information for Salivary mucin MG1 is comprised almost entirely of different glycosylated forms of the MUC5B gene product.

AFFILIATION: Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, School of Biological Sciences, 2.205, Stopford Building, Manchester M13 9PT, UK.

Country: ENGLAND

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MEDLINETA: Glycobiology

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