Ryanodine receptors in liver.

Ryanodine receptors in liver. Research Abstract Details 

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Ryanodine receptors in liver. Abstract Text:

Nicola Pierobon,Dominique C Renard-Rooney,Lawrence D Gaspers,Andrew P Thomas,

The ryanodine receptor has been mainly regarded as the Ca2+ release channel from sarcoplasmic reticulum controlling skeletal and cardiac muscle contraction. However, many studies have shown that it is widely expressed, with functions not restricted to muscular contraction. This study examined whether ryanodine receptor plays a role in calcium signaling in the liver. RT-PCR analysis of isolated hepatocytes showed expression of a truncated type 1 ryanodine receptor, but no type 2 or type 3 message was detected. We also detected binding sites for [3H]ryanodine in the microsomal cellular fraction and in permeabilized hepatocytes. This binding was displaced by caffeine and dantrolene, but not by ruthenium red, heparin or cyclic ADP-Ribose. Ryanodine, by itself, did not trigger Ca2+ oscillations in either primary cultured hepatocytes or hepatocytes within the intact perfused rat liver. In both preparations, however, ryanodine significantly increased the frequency of the cytosolic free [Ca2+] oscillations evoked by an alpha1 adrenergic receptor agonist. Experiments in permeabilized hepatocytes showed that both ryanodine and cyclic ADP-ribose evoked a slow Ca2+ leak from intracellular stores and were able to increase the Ca2+-released response to a subthreshold dose of inositol 1,4,5-trisphosphate. Our findings suggest the presence of a novel truncated form of the type 1 ryanodine receptor in rat hepatocytes. Ryanodine modulates the pattern of cytosolic free [Ca2+] oscillations by increasing oscillation frequency. We propose that the Ca2+ released from ryanodine receptors on the endoplasmic reticulum provides an increased pool of Ca2+ for positive feedback on inositol 1,4,5-trisphosphate receptors.

Ryanodine receptors in liver. Publishing Authors By Initials

N Pierobon,DC Renard-Rooney,LD Gaspers,AP Thomas,

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Ryanodine receptors in liver. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of biological chemistry

VOLUME: 281

Page Numbers: 34086-95

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 13

MONTH: 09

YEAR: 2006

Ryanodine receptors in liver. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Ryanodine receptors in liver. Keywords Mesh Terms:

KEYWORDS: Ryanodine Receptor Calcium Release Chann

MESH TERMS: metabolism

Chemical & Substance for Abstract: Ryanodine receptors in liver. Information

Substance Name: Inositol 1,4,5-Trisphosphate

Registry Number: 85166-31-0

Grant and Affiliation Information for Ryanodine receptors in liver.

AFFILIATION: Department of Pharmacology and Physiology, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: DK38422

ACRONYM: DK

MEDLINETA: J Biol Chem

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