rSac3, a novel Sac domain phosphoinositide phosphatase, promotes neurite outgrowth in PC12 cells.

rSac3, a novel Sac domain phosphoinositide phosphatase, promotes neurite outgrowth in PC12 cells. Research Abstract Details 

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rSac3, a novel Sac domain phosphoinositide phosphatase, promotes neurite outgrowth in PC12 cells. Abstract Text:

Yiyuan Yuan,Xiang Gao,Ning Guo,Hui Zhang,Zhiqin Xie,Meilei Jin,Baoming Li,Lei Yu,Naihe Jing,

Sac domain-containing proteins belong to a newly identified family of phosphoinositide phosphatases (the PIPPase family). Despite well-characterized enzymatic activity, the biological functions of this mammalian Sac domain PIPPase family remain largely unknown. We identified a novel Sac domain-containing protein, rat Sac3 (rSac3), which is widely expressed in various tissues and localized to the endoplasmic reticulum, Golgi complex and recycling endosomes. rSac3 displays PIPPase activity with PI(3)P, PI(4)P and PI(3,5)P(2) as substrates in vitro, and a mutation in the catalytic core of the Sac domain abolishes its enzymatic activity. The expression of rSac3 is upregulated during nerve growth factor (NGF)-stimulated PC12 cell neuronal differentiation, and overexpression of this protein promotes neurite outgrowth in PC12 cells. Conversely, inhibition of rSac3 expression by antisense oligonucleotides reduces neurite outgrowth of NGF-stimulated PC12 cells, and the active site mutation of rSac3 eliminates its neurite-outgrowth-promoting activity. These results indicate that rSac3 promotes neurite outgrowth in differentiating neurons through its PIPPase activity, suggesting that Sac domain PIPPase proteins may participate in forward membrane trafficking from the endoplasmic reticulum and Golgi complex to the plasma membrane, and may function as regulators of this crucial process of neuronal cell growth and differentiation.

rSac3, a novel Sac domain phosphoinositide phosphatase, promotes neurite outgrowth in PC12 cells. Publishing Authors By Initials

Y Yuan,X Gao,N Guo,H Zhang,Z Xie,M Jin,B Li,L Yu,N Jing,

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rSac3, a novel Sac domain phosphoinositide phosphatase, promotes neurite outgrowth in PC12 cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Cell research

VOLUME: 17

Page Numbers: 919-32

Journal Abbreviation: Cell Res.

ISSN: 1748-7838

DAY: 16

MONTH: Nov

YEAR: 2007

rSac3, a novel Sac domain phosphoinositide phosphatase, promotes neurite outgrowth in PC12 cells. Information

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LANGUAGE: eng

NlmUniqueID: 9425763

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Grant and Affiliation Information for rSac3, a novel Sac domain phosphoinositide phosphatase, promotes neurite outgrowth in PC12 cells.

AFFILIATION: Laboratory of Molecular Cell Biology and Key Laboratory of Stem Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue yang Road, Shanghai 200031, China.

Country: China

AGENCY: United States NIDA

GRANT: DA020555

ACRONYM: DA

MEDLINETA: Cell Res

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