Roxithromycin suppresses mucin gene expression in epithelial cells.

Roxithromycin suppresses mucin gene expression in epithelial cells. Research Abstract Details 

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Roxithromycin suppresses mucin gene expression in epithelial cells. Abstract Text:

Dong-Young Kim,Kazuhiko Takeuchi,Hajime Ishinaga,Chikako Kishioka,Shinya Suzuki,Carol Basbaum,Yuichi Majima,

Macrolide antibiotics are believed to inhibit mucus secretion but the mechanism of action is unclear. This study was designed to investigate an effect of roxithromycin on MUC2 gene expression in cultured intestinal epithelial cells (HM3-MUC2 cells). A reporter gene assay was used for analysis. Roxithromycin suppressed MUC2 gene transcriptional activity in a dose-dependent manner in HM3-MUC2 cells. Phorbol 12-myristate 13-acetate (PMA), lipoteichoic acid (LTA), lipopolysaccharide (LPS) and leukotriene D4 (LTD4) significantly increased MUC2 luciferase activities in the following order: PMA > LTA > LTD4 > LPS. Roxithromycin also decreased MUC2 gene transcriptional activity induced by PMA in a dose-dependent manner. NF-kappaB activation, but not AP-1 activation, was significantly suppressed by roxithromycin in HM3-MUC2 cells. A suppression of NF-kappaB activation was also observed in NCI-H292 cells. These results suggest that roxithromycin suppresses MUC2 gene expression in epithelial cells and that this suppression is probably via inhibition of NF-kappaB activation.

Roxithromycin suppresses mucin gene expression in epithelial cells. Publishing Authors By Initials

DY Kim,K Takeuchi,H Ishinaga,C Kishioka,S Suzuki,C Basbaum,Y Majima,

For similar genetic processes: gene expression regulation: trans-activation (genetics) research abstracts see: genetic processes: gene expression regulation: trans-activation (genetics) research

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Roxithromycin suppresses mucin gene expression in epithelial cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Pharmacology

VOLUME: 72

Page Numbers: 6-11

Journal Abbreviation: Pharmacology

ISSN: 0031-7012

DAY: 30

MONTH: Sep

YEAR: 2004

Roxithromycin suppresses mucin gene expression in epithelial cells. Information

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LANGUAGE: eng

NlmUniqueID: 152016

Roxithromycin suppresses mucin gene expression in epithelial cells. Keywords Mesh Terms:

KEYWORDS: Trans-Activation (Genetics)

MESH TERMS: drug effects

Chemical & Substance for Abstract: Roxithromycin suppresses mucin gene expression in epithelial cells. Information

Substance Name: Roxithromycin

Registry Number: 80214-83-1

Grant and Affiliation Information for Roxithromycin suppresses mucin gene expression in epithelial cells.

AFFILIATION: Department of Otorhinolaryngology, Mie University School of Medicine, Edobashi, Tsu, Japan.

Country: Switzerland

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MEDLINETA: Pharmacology

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