Roles of the amino acid side chains in the actin-binding S-site of myosin heavy chain.

Roles of the amino acid side chains in the actin-binding S-site of myosin heavy chain. Research Abstract Details 

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Roles of the amino acid side chains in the actin-binding S-site of myosin heavy chain. Abstract Text:

M Eto,R Suzuki,F Morita,H Kuwayama,N Nishi,S Tokura,

The heptapeptide Ile-Arg-Ile-Cys-Arg-Lys-Gly-OEt is the analog of the S-site, one of the actin-binding sites in myosin [Suzuki et al. (1987) J. Biol. Chem. 262, 11410-11412]. Various substituted heptapeptides were synthesized, and the dissociation constants of each acto-heptapeptide complex was measured. Comparison of the dissociation constants indicated that the hydrophobic side chain of Ile-1 was critical for the binding with F-actin, but not that of Ile-3. The positive charge and the side chain length of Arg-2 were also important. The presence of a sulfur atom in the Cys-4 was also necessary. The affinity of the N-terminal Ile-Arg-Ile part for F-actin was influenced by the kind of residues in the C-terminal tetrapeptide part. Based on these results, the side chains of Ile(702), Arg(703), and Cys(SH1)(705) in myosin subfragment-1 heavy chain were assigned to be critical for the binding with F-actin. The amino acid sequence of S-1 heavy chain containing these critical residues for the S-site from residue number 700 to 717 can be predicted as an analogue of the segment B of the ATP-binding site [Walker et al. (1982) EMBO J. 1, 945-951]. The actin-binding S-site possibly shares a part of the ATP-binding site in myosin. We discuss the possibility that the S-site is an inhibitory site of myosin ATPase and the so-called actin-activation of myosin ATPase is a deinhibition induced by transient binding of F-actin to the S-site.

Roles of the amino acid side chains in the actin-binding S-site of myosin heavy chain. Publishing Authors By Initials

M Eto,R Suzuki,F Morita,H Kuwayama,N Nishi,S Tokura,

For similar animals: chordata: vertebrates: mammals: lagomorpha: rabbits research abstracts see: animals: chordata: vertebrates: mammals: lagomorpha: rabbits research

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Roles of the amino acid side chains in the actin-binding S-site of myosin heavy chain. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 108

Page Numbers: 499-504

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 1990

Roles of the amino acid side chains in the actin-binding S-site of myosin heavy chain. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Roles of the amino acid side chains in the actin-binding S-site of myosin heavy chain. Keywords Mesh Terms:

KEYWORDS: Rabbits

MESH TERMS: metabolism

Chemical & Substance for Abstract: Roles of the amino acid side chains in the actin-binding S-site of myosin heavy chain. Information

Substance Name: Myosins

Registry Number: EC 3.6.1.4

Grant and Affiliation Information for Roles of the amino acid side chains in the actin-binding S-site of myosin heavy chain.

AFFILIATION: Department of Chemistry, Faculty of Science, Hokkaido University.

Country: JAPAN

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MEDLINETA: J Biochem

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