Roles of His-rich hpn and hpn-like proteins in Helicobacter pylori nickel physiology.

Roles of His-rich hpn and hpn-like proteins in Helicobacter pylori nickel physiology. Research Abstract Details 

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Roles of His-rich hpn and hpn-like proteins in Helicobacter pylori nickel physiology. Abstract Text:

Susmitha Seshadri, Benoit,Robert J Maier,

Individual gene-targeted hpn and hpn-like mutants and a mutant with mutations in both hpn genes were more sensitive to nickel, cobalt, and cadmium toxicity than was the parent strain, with the hpn-like strain showing the most metal sensitivity of the two individual His-rich protein mutants. The mutant strains contained up to eightfold more urease activity than the parent under nickel-deficient conditions, and the parent strain was able to achieve mutant strain activity levels by nickel supplementation. The mutants contained 3- to 4-fold more and the double mutant about 10-fold more Ni associated with their total urease pools, even though all of the strains expressed similar levels of total urease protein. Hydrogenase activities in the mutants were like those in the parent strain; thus, hydrogenase is fully activated under nickel-deficient conditions. The histidine-rich proteins appear to compete with the Ni-dependent urease maturation machinery under low-nickel conditions. Upon lowering the pH of the growth medium from 7.3 to 5, the wild-type urease activity increased threefold, but the activity in the three mutant strains was relatively unaffected. This pH effect was attributed to a nickel storage role for the His-rich proteins. Under low-nickel conditions, the addition of a nickel chelator did not significantly affect the urease activity of the wild type but decreased the activity of all of the mutants, supporting a role for the His-rich proteins as Ni reservoirs. These nickel reservoirs significantly impact the active urease activities achieved. The His-rich proteins play dual roles, as Ni storage and as metal detoxification proteins, depending on the exogenous nickel levels.

Roles of His-rich hpn and hpn-like proteins in Helicobacter pylori nickel physiology. Publishing Authors By Initials

S Seshadri,SL Benoit,RJ Maier,

For similar enzymes and coenzymes: enzymes: hydrolases: amidohydrolases: urease research abstracts see: enzymes and coenzymes: enzymes: hydrolases: amidohydrolases: urease research

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MEDLINE DATE:

Roles of His-rich hpn and hpn-like proteins in Helicobacter pylori nickel physiology. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of bacteriology

VOLUME: 189

Page Numbers: 4120-6

Journal Abbreviation: J. Bacteriol.

ISSN: 0021-9193

DAY: 23

MONTH: 03

YEAR: 2007

Roles of His-rich hpn and hpn-like proteins in Helicobacter pylori nickel physiology. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985120

Roles of His-rich hpn and hpn-like proteins in Helicobacter pylori nickel physiology. Keywords Mesh Terms:

KEYWORDS: Urease

MESH TERMS: metabolism

Chemical & Substance for Abstract: Roles of His-rich hpn and hpn-like proteins in Helicobacter pylori nickel physiology. Information

Substance Name: Urease

Registry Number: EC 3.5.1.5

Grant and Affiliation Information for Roles of His-rich hpn and hpn-like proteins in Helicobacter pylori nickel physiology.

AFFILIATION: Department of Microbiology, University of Georgia, Athens, GA 30602, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: DK06285201

ACRONYM: DK

MEDLINETA: J Bacteriol

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