Role of tyrosine-5 in the cytoplasmic tail of the macrophage asialoglycoprotein receptor in the rapid internalization of ligands.

Role of tyrosine-5 in the cytoplasmic tail of the macrophage asialoglycoprotein receptor in the rapid internalization of ligands. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Role of tyrosine-5 in the cytoplasmic tail of the macrophage asialoglycoprotein receptor in the rapid internalization of ligands. Abstract Text:

K Ozaki,N Itoh,T Kawasaki,

A macrophage asialoglycoprotein binding protein (M-ASGP-BP), which is an endocytic receptor specific for Gal/GalNAc-terminated glycoproteins, was shown to be functionally active as a homooligomer (hexamer or octamer) of a single polypeptide chain of 42 kDa [Ozaki, K., Ii, M., Itoh, N., & Kawasaki, T. (1992) J. Biol. Chem. 267, 9229-9235]. In various endocytic receptors, a four-amino-acid sequence, Tyr-X-Y-Phe, in the cytoplasmic domain has been identified as an internalization signal [Pearse, B.M.F. & Robinson, M.S. (1990) Annu. Rev. Cell Biol. 265, 151-171]. The amino-terminus of the M-ASGP-BP deduced from its cDNA sequence was found to contain the sequence, Tyr5-Glu6-Asn7-Phe8, in its cytoplasmic tail. This was confirmed by the fact that the recombinant M-ASGP-BP isolated from transfected COS-1 cells was found to have the amino-terminal sequence, Thr2-Met3-Ala4-Tyr5-Glu6-Asn7-Phe8. The role of this presumptive internalization signal in the cytoplasmic tail was studied by measuring the endocytic activity of the wild-type and mutant M-ASGP-BPs expressed on COS-1 cells through transfection with the wild-type and mutant cDNAs prepared by oligonucleotide-directed mutagenesis, respectively. On the deletion of Tyr5 or replacement of it with alanine, the internalization of asialoorosomucoid (ASOR) decreased to approximately one-fourth that in the case of the wild-type molecule. On replacement of Tyr5 with phenylalanine, the internalization proceeded at a rate similar to that in the case of the wild-type molecule.(ABSTRACT TRUNCATED AT 250 WORDS)

Role of tyrosine-5 in the cytoplasmic tail of the macrophage asialoglycoprotein receptor in the rapid internalization of ligands. Publishing Authors By Initials

K Ozaki,N Itoh,T Kawasaki,

For similar amino acids, peptides, and proteins: amino acids: amino acids, cyclic: amino acids, aromatic: tyrosine research abstracts see: amino acids, peptides, and proteins: amino acids: amino acids, cyclic: amino acids, aromatic: tyrosine research

PUBMED ID PMID:

MEDLINE DATE:

Role of tyrosine-5 in the cytoplasmic tail of the macrophage asialoglycoprotein receptor in the rapid internalization of ligands. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 113

Page Numbers: 271-6

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Mar

YEAR: 1993

Role of tyrosine-5 in the cytoplasmic tail of the macrophage asialoglycoprotein receptor in the rapid internalization of ligands. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Role of tyrosine-5 in the cytoplasmic tail of the macrophage asialoglycoprotein receptor in the rapid internalization of ligands. Keywords Mesh Terms:

KEYWORDS: Tyrosine

MESH TERMS: physiology

Chemical & Substance for Abstract: Role of tyrosine-5 in the cytoplasmic tail of the macrophage asialoglycoprotein receptor in the rapid internalization of ligands. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Role of tyrosine-5 in the cytoplasmic tail of the macrophage asialoglycoprotein receptor in the rapid internalization of ligands.

AFFILIATION: Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University.

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Role of tyrosine-5 in the cytoplasmic tail of the macrophage asialoglycoprotein receptor in the rapid internalization of ligands Related Publications

• Active transport of 5-fluorouracil and its energy coupling in Ehrlich ascites tumor cells.
• Angiotensin II type 2 receptors facilitate reinnervation of phenol-lesioned vascular calcitonin gene-related peptide-containing nerves in rat mesenteric arteries.
• Anomalous intrahepatic portal system in a patient with hilar bile duct cancer.
• Behavioral responses to jamming and 'phantom' jamming stimuli in the weakly electric fish Eigenmannia.
• Case of rapidly progressed sarcomatoid hepatocellular carcinoma in a young female without risk factor.
• Characterization of Fusarium oxysporum beta-1,6-galactanase, an enzyme that hydrolyzes larch wood arabinogalactan.
• Characterization of rat liver mannan-binding protein gene.
• Development of an in-situ X-ray diffraction system for observation of electrodeposition of metallic layers.
• Development of hepatic steatosis after pancreatoduodenectomy.
• Effects of ryanodine on permeability of choline and glucose through calcium channels in sarcoplasmic reticulum vesicles.
• Enzymatic synthesis of hydroxycinnamic acid glycerol esters using type A feruloyl esterase from Aspergillus niger.
• Evidence for in vivo synthesis of thiamin triphosphate by cytosolic adenylate kinase in chicken skeletal muscle.
• Inhibitory effect of some antibiotics on phospholipases.
• Manufacturing of microarrays.
• Mixed lineage kinase LZK forms a functional signaling complex with JIP-1, a scaffold protein of the c-Jun NH(2)-terminal kinase pathway.
• Molecular cloning and genomic analysis of mouse glucuronyltransferase involved in biosynthesis of the HNK-1 epitope.
• Multidimensional stopped-flow photometry monitoring initial processes of platelet activation.
• Properties of the active thiamine transport system in Ehrlich ascites tumor cells.
• Purification and properties of an acid phosphatase of Micrococcus denitrificans distinct from thiamine phosphate phosphatase.
• Purification and properties of thiamine pyrophosphokinase in Paracoccus denitrificans.
• Reaction mechanism underlying CMP-N-acetylneuraminic acid hydroxylation in mouse liver: formation of a ternary complex of cytochrome b5, CMP-N-acetylneuraminic acid, and a hydroxylation enzyme.
• Role of tyrosine-5 in the cytoplasmic tail of the macrophage asialoglycoprotein receptor in the rapid internalization of ligands.
• Stimulus selectivity is enhanced by voltage-dependent conductances in combination-sensitive neurons.
• Studies on the binding of thiamine pyrophosphate to apoenzyme of yeast pyruvate decarboxylase.
• Two forms of messenger RNA encoding rat liver mannan-binding protein are generated by differential utilization of polyadenylation sites of one transcript.
• Vacuum ultraviolet photodissociation and surface morphology change of water ice films dosed with hydrogen chloride.
• [Effect of long-term treatment with royal jelly on insulin resistance in Otsuka Long-Evans Tokushima Fatty (OLETF) rats]
• [Effect of propolis on insulin resistance in fructose-drinking rats]
• [Hypertension in young adults]
• [Surgically induced astigmatism after micro and small clear temporal corneal incision in cataract surgery]