Role of the mutant spectrum in adaptation and replication of West Nile virus.

Role of the mutant spectrum in adaptation and replication of West Nile virus. Research Abstract Details 

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Role of the mutant spectrum in adaptation and replication of West Nile virus. Abstract Text:

Alexander T Ciota,Kiet A Ngo,Amy O Lovelace,Anne F Payne,Yangsheng Zhou,Pei-Yong Shi,Laura D Kramer,

West Nile virus (WNV) has successfully spread throughout the USA, Canada, Mexico, the Caribbean and parts of Central and South America since its 1999 introduction into North America. Despite infecting a broad range of both mosquito and avian species, the virus remains highly genetically conserved. This lack of evolutionary change over space and time is common with many arboviruses and is frequently attributed to the adaptive constraints resulting from the virus cycling between vertebrate hosts and invertebrate vectors. WNV, like most RNA viruses studied thus far, has been shown in nature to exist as a highly genetically diverse population of genotypes. Few studies have directly evaluated the role of these mutant spectra in viral fitness and adaptation. Using clonal analysis and reverse genetics experiments, this study evaluated genotype diversity and the importance of consensus change in producing the adaptive phenotype of WNV following sequential mosquito cell passage. The results indicated that increases in the replicative ability of WNV in mosquito cells correlate with increases in the size of the mutant spectrum, and that consensus change is not solely responsible for alterations in viral fitness and adaptation of WNV. These data provide evidence of the importance of quasispecies dynamics in the adaptation of a flavivirus to new and changing environments and hosts, with little evidence of significant genetic change.

Role of the mutant spectrum in adaptation and replication of West Nile virus. Publishing Authors By Initials

AT Ciota,KA Ngo,AO Lovelace,AF Payne,Y Zhou,PY Shi,LD Kramer,

For similar viruses: rna viruses: flaviviridae: flavivirus: encephalitis viruses, japanese: west nile virus research abstracts see: viruses: rna viruses: flaviviridae: flavivirus: encephalitis viruses, japanese: west nile virus research

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MEDLINE DATE:

Role of the mutant spectrum in adaptation and replication of West Nile virus. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of general virology

VOLUME: 88

Page Numbers: 865-74

Journal Abbreviation: J. Gen. Virol.

ISSN: 0022-1317

DAY: 3

MONTH: Mar

YEAR: 2007

Role of the mutant spectrum in adaptation and replication of West Nile virus. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 77340

Role of the mutant spectrum in adaptation and replication of West Nile virus. Keywords Mesh Terms:

KEYWORDS: West Nile virus

MESH TERMS: physiology

Chemical & Substance for Abstract: Role of the mutant spectrum in adaptation and replication of West Nile virus. Information

Substance Name: RNA, Viral

Registry Number: 0

Grant and Affiliation Information for Role of the mutant spectrum in adaptation and replication of West Nile virus.

AFFILIATION: Wadsworth Center, New York State Department of Health, Albany, NY 12201, USA. aciota@wadsworth.org

Country: England

AGENCY: United States NIAID

GRANT: R01-AI-50758

ACRONYM: AI

MEDLINETA: J Gen Virol

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