Role of the ETB receptor in retinal ganglion cell death in glaucoma.

Role of the ETB receptor in retinal ganglion cell death in glaucoma. Research Abstract Details 

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Role of the ETB receptor in retinal ganglion cell death in glaucoma. Abstract Text:

Recent observations suggest that the vasoactive peptide endothelin-1 (ET-1) may be an important contributor to the etiology of glaucoma. ET-1 administration has been shown to produce optic nerve axonal loss and apoptosis of retinal ganglion cells. Ocular ET-1 levels are elevated in aqueous humor in response to elevated intraocular pressure both in glaucoma patients and in animal models of glaucoma; however, the precise mechanisms by which ET-1 mediates glaucomatous optic neuropathy are not clear. Presently we report that ET-1-mediated apoptosis was markedly attenuated in ETB receptor-deficient rats, suggesting a key role for ETB receptors in apoptosis of retinal ganglion cells by ET-1 treatment. Using virally transformed rat retinal ganglion cells (RGC-5 cells), we found that ET-1 (100 nmol/L) treatment produced apoptotic changes in these cells that was determined by flow cytometric analyses, release of mitochondrial cytochrome c to the cytosol, and increased phosphorylation of c-Jun N-terminal kinase. Pretreatment with the ETB-receptor antagonist BQ788 (1 micromol/L) was able to significantly attenuate ET-1-mediated apoptosis in RGC-5 cells. ET-1-mediated apoptotic changes in RGC-5 cells were associated with ETB-receptor activation and were accompanied by a significant upregulation of ETB-receptor expression. These studies suggest that ocular ET-1 acts through ETB receptors to mediate apoptosis of retinal ganglion cells, a key event in glaucoma and related optic neuropathies.

Role of the ETB receptor in retinal ganglion cell death in glaucoma. Publishing Authors By Initials

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Role of the ETB receptor in retinal ganglion cell death in glaucoma. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Canadian journal of physiology and pharmacology

VOLUME: 86

Page Numbers: 380-93

Journal Abbreviation: Can. J. Physiol. Pharmacol.

ISSN: 0008-4212

DAY: 26

MONTH: Jun

YEAR: 2008

Role of the ETB receptor in retinal ganglion cell death in glaucoma. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 372712

Role of the ETB receptor in retinal ganglion cell death in glaucoma. Keywords Mesh Terms:

KEYWORDS: Up-Regulation

MESH TERMS: metabolism

Chemical & Substance for Abstract: Role of the ETB receptor in retinal ganglion cell death in glaucoma. Information

Substance Name: JNK Mitogen-Activated Protein Kinases

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Role of the ETB receptor in retinal ganglion cell death in glaucoma.

AFFILIATION: Department of Pharmacology and Neuroscience, UNT Health Science Center, 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA. rkrishna@hsc.unt.edu

Country: Canada

AGENCY: United States NEI

GRANT: R01 EY11979

ACRONYM: EY

MEDLINETA: Can J Physiol Pharmacol

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