Role of protein O-mannosyltransferase Pmt4 in the morphogenesis and virulence of Cryptococcus neoformans.

Role of protein O-mannosyltransferase Pmt4 in the morphogenesis and virulence of Cryptococcus neoformans. Research Abstract Details 

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Role of protein O-mannosyltransferase Pmt4 in the morphogenesis and virulence of Cryptococcus neoformans. Abstract Text:

Gillian M Olson,Deborah S Fox,Ping Wang,J Andrew Alspaugh,Kent L Buchanan,

Protein O mannosylation is initiated in the endoplasmic reticulum by protein O-mannosyltransferases (Pmt proteins) and plays an important role in the secretion, localization, and function of many proteins, as well as in cell wall integrity and morphogenesis in fungi. Three Pmt proteins, each belonging to one of the three respective Pmt subfamilies, are encoded in the genome of the human fungal pathogen Cryptococcus neoformans. Disruption of the C. neoformans PMT4 gene resulted in abnormal growth morphology and defective cell separation. Transmission electron microscopy revealed defective cell wall septum degradation during mother-daughter cell separation in the pmt4 mutant compared to wild-type cells. The pmt4 mutant also demonstrated sensitivity to elevated temperature, sodium dodecyl sulfate, and amphotericin B, suggesting cell wall defects. Further analysis of cell wall protein composition revealed a cell wall proteome defect in the pmt4 mutant, as well as a global decrease in protein mannosylation. Heterologous expression of C. neoformans PMT4 in a Saccharomyces cerevisiae pmt1pmt4 mutant strain functionally complemented the deficient Pmt activity. Furthermore, Pmt4 activity in C. neoformans was required for full virulence in two murine models of disseminated cryptococcal infection. Taken together, these results indicate a central role for Pmt4-mediated protein O mannosylation in growth, cell wall integrity, and virulence of C. neoformans.

Role of protein O-mannosyltransferase Pmt4 in the morphogenesis and virulence of Cryptococcus neoformans. Publishing Authors By Initials

GM Olson,DS Fox,P Wang,JA Alspaugh,KL Buchanan,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: virulence research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: virulence research

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Role of protein O-mannosyltransferase Pmt4 in the morphogenesis and virulence of Cryptococcus neoformans. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Eukaryotic cell

VOLUME: 6

Page Numbers: 222-34

Journal Abbreviation: Eukaryotic Cell

ISSN: 1535-9778

DAY: 1

MONTH: 12

YEAR: 2006

Role of protein O-mannosyltransferase Pmt4 in the morphogenesis and virulence of Cryptococcus neoformans. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101130731

Role of protein O-mannosyltransferase Pmt4 in the morphogenesis and virulence of Cryptococcus neoformans. Keywords Mesh Terms:

KEYWORDS: Virulence

MESH TERMS: physiology

Chemical & Substance for Abstract: Role of protein O-mannosyltransferase Pmt4 in the morphogenesis and virulence of Cryptococcus neoformans. Information

Substance Name: protein O-mannosyltransferase

Registry Number: EC 2.4.1.109

Grant and Affiliation Information for Role of protein O-mannosyltransferase Pmt4 in the morphogenesis and virulence of Cryptococcus neoformans.

AFFILIATION: Department of Microbiology and Immunology, Tulane University Health Sciences Center, New Orleans, Louisiana, USA. golson@chnola-Research.org

Country: United States

AGENCY: United States NIAID

GRANT: AI055302

ACRONYM: AI

MEDLINETA: Eukaryot Cell

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