Role of p38 MAPK in lupeol-induced B16 2F2 mouse melanoma cell differentiation.

Role of p38 MAPK in lupeol-induced B16 2F2 mouse melanoma cell differentiation. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Role of p38 MAPK in lupeol-induced B16 2F2 mouse melanoma cell differentiation. Abstract Text:

Keishi Hata,Kazuyuki Hori,Saori Takahashi,

We examined the signaling mechanisms involved in the differentiation-inducing activity of lupeol toward B16 2F2 melanoma cells. alpha-Melanocyte stimulating hormone (alpha-MSH), forskolin and dibutyryl cAMP, which are believed to be cAMP-elevating agents and analogues, enhanced lupeol-induced B16 2F2 cell differentiation. However, H89, an inhibitor of protein kinase A, completely abolished B16-2F2 cell differentiation induced by lupeol. Furthermore, we studied the role of mitogen-activated protein kinases (MAPKs) in lupeol-induced B16 2F2 cell differentiation. U0126, an inhibitor of MAPK kinases, induced B16 2F2 cell differentiation and enhanced the cell differentiation induced by lupeol. However, SB203580, a selective inhibitor of p38 MAPK, completely blocked lupeol-induced B16 2F2 cell differentiation. Western blot analysis revealed that 10 microM lupeol transiently elevated the level of phosphorylation of p38 MAPK. The phosphorylation of p38 MAPK was detected on the addition of 1 microM lupeone, another lupane triterpene, but was not induced by 1 microM lupeol. These results suggested that lupeol induced B16 2F2 cell differentiation through activation of p38 MAPK, and that the structural differences at C-3 of lupane triterpenes played an important role in the activation of p38 MAPK.

Role of p38 MAPK in lupeol-induced B16 2F2 mouse melanoma cell differentiation. Publishing Authors By Initials

K Hata,K Hori,S Takahashi,

For similar enzymes and coenzymes: enzymes: transferases: phosphotransferases: phosphotransferases (alcohol group acceptor): protein kinases: protein-serine-threonine kinases: mitogen-activated protein kinases: p38 mitogen-activated protein kinases research abstracts see: enzymes and coenzymes: enzymes: transferases: phosphotransferases: phosphotransferases (alcohol group acceptor): protein kinases: protein-serine-threonine kinases: mitogen-activated protein kinases: p38 mitogen-activated protein kinases research

PUBMED ID PMID:

MEDLINE DATE:

Role of p38 MAPK in lupeol-induced B16 2F2 mouse melanoma cell differentiation. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 134

Page Numbers: 441-5

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 2003

Role of p38 MAPK in lupeol-induced B16 2F2 mouse melanoma cell differentiation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Role of p38 MAPK in lupeol-induced B16 2F2 mouse melanoma cell differentiation. Keywords Mesh Terms:

KEYWORDS: p38 Mitogen-Activated Protein Kinases

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Role of p38 MAPK in lupeol-induced B16 2F2 mouse melanoma cell differentiation. Information

Substance Name: p38 Mitogen-Activated Protein Kinases

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Role of p38 MAPK in lupeol-induced B16 2F2 mouse melanoma cell differentiation.

AFFILIATION: Department of Bioengineering, Akita Research Institute of Food & Brewing (ARIF), 4-26 Sanuki, Araya-machi, Akita 010-1623.

Country: Japan

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Role of p38 MAPK in lupeol-induced B16 2F2 mouse melanoma cell differentiation Related Publications

• A noncanonical E-box enhancer drives mouse Period2 circadian oscillations in vivo.
• Bone morphogenetic protein 2 stimulates osteoclast differentiation and survival supported by receptor activator of nuclear factor-kappaB ligand.
• Circadian rhythms: the cancer connection.
• Comparison between stabilometry with and without head tilts in a roll plane.
• Effect of Escherichia coli wild type or its derivative with high nitrite reductase activity on in vitro ruminal methanogenesis and nitrate/nitrite reduction.
• Effect of gravity on apical dominance in Pharbitis nil.
• Effect of oral branched chain amino acid-rich nutrient administered during endoscopic injection sclerotherapy of cirrhotic patients.
• Forward genetic screens to identify circadian rhythm mutants in mice.
• Genome-wide epistatic interaction analysis reveals complex genetic determinants of circadian behavior in mice.
• Identification of the cDNA of differentially expressed genes in the transition zone of cucumber seedlings by fluorescent differential display.
• In vivo bone-forming capacity of human bone marrow-derived stromal cells is stimulated by recombinant human bone morphogenetic protein-2.
• Isolation of auxin efflux carrier cDNA from cucumber.
• Methods to record circadian rhythm wheel running activity in mice.
• Mice lacking smooth muscle calponin display increased bone formation that is associated with enhancement of bone morphogenetic protein responses.
• Mouse chimeras and their application to circadian biology.
• Novel hydrotropism mutants of Arabidopsis thaliana and their altered waving response and phototropism.
• Oral myiasis treated with ivermectin: case report.
• Osteoblasts provide a suitable microenvironment for the action of receptor activator of nuclear factor-kappaB ligand.
• Osteoprotegerin regulates bone formation through a coupling mechanism with bone resorption.
• Pax 2 expression in mesodermal segmentation and its relationship with EphA4 and Lunatic-fringe during chicken somitogenesis.
• Physiological and genetic characterization of hydrotropic mutants of Arabidopsis thaliana.
• Real-time luminescence reporting of circadian gene expression in mammals.
• Regulations: what next?
• The effect of light on the gravimorphogenesis of cucumber seedlings.
• The mouse Clock mutation reduces circadian pacemaker amplitude and enhances efficacy of resetting stimuli and phase-response curve amplitude.
• [Bone morphogenetic protein (BMP): from basic studies to clinical approaches]
• [Differential expression of a homing-related molecule repertoire among umbilical cord blood, mobilized peripheral blood and bone marrow-derived hematopoietic stem/progenitor cells.]
• [Gravitaxis of paramecium: testing the hypotheses by free-fall experiments]
• [Sequence variability in Borna disease virus ORF II from human beings]
• [The effects of space environment on differentiation and mutation frequency in Dictyostelium discoideum]