Role of NHE1 in calcium signaling and cell proliferation in human CNS pericytes.

Role of NHE1 in calcium signaling and cell proliferation in human CNS pericytes. Research Abstract Details 

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Role of NHE1 in calcium signaling and cell proliferation in human CNS pericytes. Abstract Text:

Kuniyuki Nakamura,Masahiro Kamouchi,Takanari Kitazono,Junya Kuroda,Ryu Matsuo,Noriko Hagiwara,Eiichi Ishikawa,Hiroaki Ooboshi,Setsuro Ibayashi,Mitsuo Iida,

The central nervous system (CNS) pericytes play an important role in brain microcirculation. Na(+)/H(+) exchanger isoform 1 (NHE1) has been suggested to regulate the proliferation of nonvascular cells through the regulation of intracellular pH, Na(+), and cell volume; however, the relationship between NHE1 and intracellular Ca(2+), an essential signal of cell growth, is still not known. The aim of the present study was to elucidate the role of NHE1 in Ca(2+) signaling and the proliferation of human CNS pericytes. The intracellular Ca(2+) concentration was measured by fura 2 in cultured human CNS pericytes. The cells showed spontaneous Ca(2+) oscillation under quasi-physiological ionic conditions. A decrease in extracellular pH or Na(+) evoked a transient Ca(2+) rise followed by Ca(2+) oscillation, whereas an increase in pH or Na(+) did not induce the Ca(2+) responses. The Ca(2+) oscillation was inhibited by an inhibitor of NHE in a dose-dependent manner and by knockdown of NHE1 by using RNA interference. The Ca(2+) oscillation was completely abolished by thapsigargin. The proliferation of pericytes was attenuated by inhibition of NHE1. These results demonstrate that NHE1 regulates Ca(2+) signaling via the modulation of Ca(2+) release from the endoplasmic reticulum, thus contributing to the regulation of proliferation in CNS pericytes.

Role of NHE1 in calcium signaling and cell proliferation in human CNS pericytes. Publishing Authors By Initials

K Nakamura,M Kamouchi,T Kitazono,J Kuroda,R Matsuo,N Hagiwara,E Ishikawa,H Ooboshi,S Ibayashi,M Iida,

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Role of NHE1 in calcium signaling and cell proliferation in human CNS pericytes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: American journal of physiology. Heart and circulat

VOLUME: 294

Page Numbers: H1700-7

Journal Abbreviation: Am. J. Physiol. Heart Circ. Ph

ISSN: 0363-6135

DAY: 8

MONTH: 02

YEAR: 2008

Role of NHE1 in calcium signaling and cell proliferation in human CNS pericytes. Information

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LANGUAGE: eng

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AFFILIATION: Dept. of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu Univ., Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan. kamouchi@intmed2.med.kyushu-u.ac.jp).

Country: United States

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MEDLINETA: Am J Physiol Heart Circ Physio

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