Role of myosin II activity and the regulation of myosin light chain phosphorylation in astrocytomas.

Role of myosin II activity and the regulation of myosin light chain phosphorylation in astrocytomas. Research Abstract Details 

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Role of myosin II activity and the regulation of myosin light chain phosphorylation in astrocytomas. Abstract Text:

Bodour Salhia,Jeong Hyun Hwang,Christian A Smith,Mitsutoshi Nakada,Fiona Rutka,Marc Symons,James T Rutka,Bodour Salhia,Jeong Hyun Hwang,Christian A Smith,Mitsutoshi Nakada,Fiona Rutka,Marc Symons,James T Rutka,

The generation of contractile force mediated by actin-myosin interactions is essential for cell motility. Myosin activity is promoted by phosphorylation of myosin light chain (MLC). MLC phosphorylation in large part is controlled by kinases that are effectors of Rho family GTPases. Accordingly, in this study we examined the effects of ROCK and Rac1 inhibition on MLC phosphorylation in astrocytoma cells. We found that low concentrations of the ROCK inhibitor Y27632 increased the phosphorylation state of the Triton X-100 soluble fraction of MLC, whereas higher concentrations of Y27632 decreased soluble phospho-MLC. These effects of Y27632 were dependent on Rac1. The soluble form of phospho-MLC comprises about 10% of total phospho-MLC in control cells. Interestingly, ROCK inhibition led to a decrease in the phosphorylation state of total MLC, whereas Rac1 inhibition had little effect. Thus, the soluble form of MLC is differentially regulated by ROCK and Rac1 compared with MLC examined in a total cell extract. We also observed that astrocytoma migration is stimulated by low concentrations of the myosin II inhibitor blebbistatin. However, higher concentrations of blebbistatin inhibit migration leading us to believe that migration has a biphasic dependence on myosin II activity. Taken together, our data show that modulation of myosin II activity is important in determining optimal astrocytoma migration. In addition, these findings suggest that there are at least two populations of MLC that are differentially regulated. Cell Motil. Cytoskeleton 2008. (c) 2007 Wiley-Liss, Inc.

Role of myosin II activity and the regulation of myosin light chain phosphorylation in astrocytomas. Publishing Authors By Initials

B Salhia,JH Hwang,CA Smith,M Nakada,F Rutka,M Symons,JT Rutka,B Salhia,JH Hwang,CA Smith,M Nakada,F Rutka,M Symons,JT Rutka,

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Role of myosin II activity and the regulation of myosin light chain phosphorylation in astrocytomas. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Cell motility and the cytoskeleton

VOLUME: 65

Page Numbers: 12-24

Journal Abbreviation: Cell Motil. Cytoskeleton

ISSN: 0886-1544

DAY: 24

MONTH: Jan

YEAR: 2008

Role of myosin II activity and the regulation of myosin light chain phosphorylation in astrocytomas. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Role of myosin II activity and the regulation of myosin light chain phosphorylation in astrocytomas.

AFFILIATION: The Arthur and Sonia Labatt Brain Tumor Research Center, The Hospital for Sick Children, The University of Toronto, Toronto, Ontario, Canada.

Country: United States

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MEDLINETA: Cell Motil Cytoskeleton

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