Role of mRNA stability in growth phase regulation of gene expression in the group A streptococcus.

Role of mRNA stability in growth phase regulation of gene expression in the group A streptococcus. Research Abstract Details 

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Role of mRNA stability in growth phase regulation of gene expression in the group A streptococcus. Abstract Text:

Timothy C Barnett,Julia V Bugrysheva,June R Scott,

The impressive disease spectrum of Streptococcus pyogenes (the group A streptococcus [GAS]) is believed to be determined by its ability to modify gene expression in response to environmental stimuli. Virulence gene expression is controlled tightly by several different transcriptional regulators in this organism. In addition, expression of most, if not all, GAS genes is determined by a global mechanism dependent on growth phase. To begin an analysis of growth-phase regulation, we compared the transcriptome 2 h into stationary phase to that in late exponential phase of a serotype M3 GAS strain. We identified the arc transcript as more abundant in stationary phase in addition to the sag and sda transcripts that had been previously identified. We found that in stationary phase, the stability of sagA, sda, and arcT transcripts increased dramatically. We found that polynucleotide phosphorylase (PNPase [encoded by pnpA]) is rate limiting for decay of sagA and sda transcripts in late exponential phase, since the stability of these mRNAs was greater in a pnpA mutant, while stability of control mRNAs was unaffected by this mutation. Complementation restored the wild-type decay rate. Furthermore, in a pnpA mutant, the sagA mRNA appeared to be full length, as determined by Northern hybridization. It seems likely that mRNAs abundant in stationary phase are insensitive to the normal decay enzyme(s) and instead require PNPase for this process. It is possible that PNPase activity is limited in stationary phase, allowing persistence of these important virulence factor transcripts at this phase of growth.

Role of mRNA stability in growth phase regulation of gene expression in the group A streptococcus. Publishing Authors By Initials

TC Barnett,JV Bugrysheva,JR Scott,

For similar genetic processes: gene expression: transcription, genetic research abstracts see: genetic processes: gene expression: transcription, genetic research

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Role of mRNA stability in growth phase regulation of gene expression in the group A streptococcus. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of bacteriology

VOLUME: 189

Page Numbers: 1866-73

Journal Abbreviation: J. Bacteriol.

ISSN: 0021-9193

DAY: 22

MONTH: 12

YEAR: 2006

Role of mRNA stability in growth phase regulation of gene expression in the group A streptococcus. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985120

Role of mRNA stability in growth phase regulation of gene expression in the group A streptococcus. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: growth & development

Chemical & Substance for Abstract: Role of mRNA stability in growth phase regulation of gene expression in the group A streptococcus. Information

Substance Name: Polyribonucleotide Nucleotidyltransferas

Registry Number: EC 2.7.7.8

Grant and Affiliation Information for Role of mRNA stability in growth phase regulation of gene expression in the group A streptococcus.

AFFILIATION: Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322, USA.

Country: United States

AGENCY: United States NIAID

GRANT: R01-AI20723

ACRONYM: AI

MEDLINETA: J Bacteriol

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