Role of metal ions in the tetraloop-receptor complex as analyzed by NMR.

Role of metal ions in the tetraloop-receptor complex as analyzed by NMR. Research Abstract Details 

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Role of metal ions in the tetraloop-receptor complex as analyzed by NMR. Abstract Text:

Jared H Davis,Trenton R Foster,Marco Tonelli,Samuel E Butcher,

Metal ions are critical for the proper folding of RNA, and the GAAA tetraloop-receptor is necessary for the optimal folding and function of many RNAs. We have used NMR to investigate the role of metal ions in the structure of the tetraloop-receptor in solution. The NMR data indicate native tertiary structure is formed under a wide range of ionic conditions. The lack of conformational adaptation in response to very different ionic conditions argues against a structural role for divalent ions. Nuclear Overhauser effects to cobalt hexammine and paramagnetic relaxation enhancement induced by manganese ions were used to determine the NMR structures of the tetraloop receptor in association with metal ions, providing the first atomic-level view of these interactions in the solution state. Five manganese and two cobalt hexammine ions could be localized to the RNA surface. The locations of the associated metal ions are similar, but not identical to, those of previously determined crystal structures. The sites of association are in general agreement with nonlinear Poisson-Boltzmann calculations of the electrostatic surface, emphasizing the general importance of diffusely associated ions in RNA tertiary structure.

Role of metal ions in the tetraloop-receptor complex as analyzed by NMR. Publishing Authors By Initials

JH Davis,TR Foster,M Tonelli,SE Butcher,

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MEDLINE DATE:

Role of metal ions in the tetraloop-receptor complex as analyzed by NMR. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: RNA (New York, N.Y.)

VOLUME: 13

Page Numbers: 76-86

Journal Abbreviation: RNA

ISSN: 1355-8382

DAY: 21

MONTH: 11

YEAR: 2006

Role of metal ions in the tetraloop-receptor complex as analyzed by NMR. Information

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LANGUAGE: eng

NlmUniqueID: 9509184

Role of metal ions in the tetraloop-receptor complex as analyzed by NMR. Keywords Mesh Terms:

KEYWORDS: Solutions

MESH TERMS: chemistry

Chemical & Substance for Abstract: Role of metal ions in the tetraloop-receptor complex as analyzed by NMR. Information

Substance Name: Cobalt

Registry Number: 7440-48-4

Grant and Affiliation Information for Role of metal ions in the tetraloop-receptor complex as analyzed by NMR.

AFFILIATION: Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: T32 GM08293

ACRONYM: GM

MEDLINETA: RNA

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