Role of hepatocyte growth factor/c-Met signaling in regulating urokinase plasminogen activator on invasiveness in human hepatocellular carcinoma: a potential therapeutic target.

Role of hepatocyte growth factor/c-Met signaling in regulating urokinase plasminogen activator on invasiveness in human hepatocellular carcinoma: a potential therapeutic target. Research Abstract Details 

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Role of hepatocyte growth factor/c-Met signaling in regulating urokinase plasminogen activator on invasiveness in human hepatocellular carcinoma: a potential therapeutic target. Abstract Text:

Kyung Hee Lee,Eun Young Choi,Myung Soo Hyun,Byung Ik Jang,Tae Nyeun Kim,Heon Ju Lee,Jong Yuel Eun,Hong Gin Kim,Sung Soo Yoon,Dong Sik Lee,Jung Hye Kim,Jae-Ryong Kim,

Hepatocyte growth factor (HGF), its transmembrane tyrosine kinase receptor (c-Met), and urokinase type plasminogen activator (uPA) is a key protein in the plasminogen activation system, which plays a proteolytically important role in the invasion and metastasis of various types of cancers. However, the mechanisms by which HGF/c-Met signaling mediates cancer progression and metastasis are unclear. This study was designed to investigate the roles of HGF/c-Met in tumor progression and metastasis in HepG2 and Hep3B hepatoma cell lines. Treatment with HGF increased c-Met phosphorylation in a dose-dependent manner. Activity of c-Met phosphorylation peaked 1-3 min after HGF treatment and then declined. HGF enhanced the protein level and the activity of uPA in HepG2 and Hep3B cells, and the uPAR protein level also increased in a HGF dose-dependent manner. HGF increased cell invasion through the Matrigel. A monoclonal antibody against human uPA receptor, mAb 3936, inhibited HGF-mediated tumor cell invasion in a dose-dependent manner. Down-regulation of uPA using uPA-shRNA induced a decrease in in vitro cell invasion. These results suggest that hepatoma cells express functional c-Met, which may provide a target for a therapeutic basis to interfere with metastases of cancer cells by inhibiting uPA system-mediated proteolysis.

Role of hepatocyte growth factor/c-Met signaling in regulating urokinase plasminogen activator on invasiveness in human hepatocellular carcinoma: a potential therapeutic target. Publishing Authors By Initials

KH Lee,EY Choi,MS Hyun,BI Jang,TN Kim,HJ Lee,JY Eun,HG Kim,SS Yoon,DS Lee,JH Kim,JR Kim,

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Role of hepatocyte growth factor/c-Met signaling in regulating urokinase plasminogen activator on invasiveness in human hepatocellular carcinoma: a potential therapeutic target. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Clinical & experimental metastasis

VOLUME: 25

Page Numbers: 89-96

Journal Abbreviation: Clin. Exp. Metastasis

ISSN: 0262-0898

DAY: 9

MONTH: 11

YEAR: 2007

Role of hepatocyte growth factor/c-Met signaling in regulating urokinase plasminogen activator on invasiveness in human hepatocellular carcinoma: a potential therapeutic target. Information

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LANGUAGE: eng

NlmUniqueID: 8409970

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Grant and Affiliation Information for Role of hepatocyte growth factor/c-Met signaling in regulating urokinase plasminogen activator on invasiveness in human hepatocellular carcinoma: a potential therapeutic target.

AFFILIATION: Department of Hemato-Oncology, College of Medicine, Yeungnam University, Daegu, Republic of Korea.

Country: Netherlands

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MEDLINETA: Clin Exp Metastasis

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