Role of glucose in cloned mouse embryo development.

Role of glucose in cloned mouse embryo development. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Role of glucose in cloned mouse embryo development. Abstract Text:

Cloned mouse embryos display a marked preference for glucose-containing culture medium, with enhanced development to the blastocyst stage in glucose-containing medium attributable mainly to an early beneficial effect during the first cell cycle. This early beneficial effect of glucose is not displayed by parthenogenetic, fertilized, or tetraploid nuclear transfer control embryos, indicating that it is specific to diploid clones. Precocious localization of the glucose transporter SLC2A1 to the cell surface, as well as increased expression of glucose transporters and increased uptake of glucose at the one- and two-cell stages, is also seen in cloned embryos. To examine the role of glucose in early cloned embryo development, we examined glucose metabolism and associated metabolites, as well as mitochondrial ultrastructure, distribution, and number. Clones prepared with cumulus cell nuclei displayed significantly enhanced glucose metabolism at the two-cell stage relative to parthenogenetic controls. Despite the increase in metabolism, ATP content was reduced in clones relative to parthenotes and fertilized controls. Clones at both stages displayed elevated concentrations of glycogen compared with parthenogenetic controls. There was no difference in the number of mitochondria, but clone mitochondria displayed ultrastructural alterations. Interestingly, glucose availability positively affected mitochondrial structure and localization. We conclude that cloned embryos may be severely compromised in terms of ATP-dependent processes during the first two cell cycles and that glucose may exert its early beneficial effects via positive effects on the mitochondria.

Role of glucose in cloned mouse embryo development. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Role of glucose in cloned mouse embryo development. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: American journal of physiology. Endocrinology and

VOLUME: 295

Page Numbers: E798-809

Journal Abbreviation:

ISSN: 0193-1849

DAY: 24

MONTH: 06

YEAR: 2008

Role of glucose in cloned mouse embryo development. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901226

Role of glucose in cloned mouse embryo development. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Role of glucose in cloned mouse embryo development. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Role of glucose in cloned mouse embryo development.

AFFILIATION: 3307 North Broad St., Philadelphia, PA 19140. klatham@temple.edu).

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Am J Physiol Endocrinol Metab

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Role of glucose in cloned mouse embryo development Related Publications

• A balanced memory network.
• A case of unilateral cerebellar degeneration, with a note on the Golgi type II neurons.
• A new concept of the early maxillary growth mechanism.
• A theoretical assessment of a proposed technique of stereo-tomography.
• Analysis of polysomal mRNA populations of mouse oocytes and zygotes: dynamic changes in maternal mRNA utilization and function.
• Chapter 7 oocyte quality and maternal control of development.
• Chronic osteomyelitis. The effect of the extent of surgical resection on infection-free survival.
• Corrosion of cemented titanium femoral stems.
• Deficiency in recapitulation of stage-specific embryonic gene transcription in two-cell stage cloned mouse embryos.
• Differential expression of cell cycle genes in rhesus monkey oocytes and embryos of different developmental potentials.
• Dilution effects in a spirit-stored syringe.
• Does functionally based activity make a difference to health status and mobility? A randomised controlled trial in residential care facilities (The Promoting Independent Living Study; PILS).
• Effects of in vitro oocyte maturation and embryo culture on the expression of glucose transporters, glucose metabolism and insulin signaling genes in rhesus monkey oocytes and preimplantation embryos.
• Erratum: Deficiency in recapitulation of stage-specific embryonic gene transcription in two-cell stage cloned mouse embryos.
• Good sense over dollars and cents.
• HAZARDS OF GROUNDNUTS.
• Hybrid vigor and transgenerational epigenetic effects on early mouse embryo phenotype.
• Infant feeding practices in St. Vincent and factors which affect them.
• Nonverbal arithmetic in humans: light from noise.
• Optimization of procedures for cloning by somatic cell nuclear transfer in mice.
• Radiographic appearances in lumbar disc prolapse.
• Role of glucose in cloned mouse embryo development.
• Sea-going hardware for the cloud albedo method of reversing global warming.
• Spontaneous fractures of pelvis in rheumatoid arthritis.
• Syngamus in an Australian.
• The Primate Embryo Gene Expression Resource in embryology and stem cell biology.
• The oxygen paradox. Experiments on the effects of oxygen in human anoxia.
• The training of home helps.
• Therapeutic cloning: status and prospects.
• Unstable resonators with 90 degrees beam rotation.