Role of GldA in dihydroxyacetone and methylglyoxal metabolism of Escherichia coli K12.

Role of GldA in dihydroxyacetone and methylglyoxal metabolism of Escherichia coli K12. Research Abstract Details 

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Role of GldA in dihydroxyacetone and methylglyoxal metabolism of Escherichia coli K12. Abstract Text:

Krishna P Subedi,Insook Kim,Junho Kim,Bumchan Min,Chankyu Park,Krishna P Subedi,Insook Kim,Junho Kim,Bumchan Min,Chankyu Park,

The metabolic pathway involving dihydroxyacetone is poorly characterized although novel enzymes associated with this metabolite have recently been demonstrated. The role of GldA in dihydroxyacetone and methylglyoxal metabolism was investigated by purifying the enzyme and characterizing its catalytic ability using nuclear magnetic resonance (NMR) spectroscopy. At neutral pH, the enzyme exhibits much higher affinities towards dihydroxyacetone, methylglyoxal, and glycolaldehyde than glycerol with K(m) values of 0.30, 0.50, 0.85, and 56 mM, respectively. This is consistent with NMR data with crude extracts, showing that the conversion from dihydroxyacetone to glycerol by GldA is far more efficient than the reverse reaction. Dihydroxyacetone was found to be lethal at higher concentration with an LC(50) value of 28 mM compared with 0.4 mM of methylglyoxal, while lactaldehyde was found to exhibit significant growth inhibition in Escherichia coli cells. The toxicity of dihydroxyacetone appears to be due to its intracellular conversion to an aldehyde compound, presumably methylglyoxal, since the glyoxalase mutant becomes sensitive to dihydroxyacetone. Based on information that gldA is preceded in an operon by the ptsA homolog and talC gene encoding fructose 6-phosphate aldolase, this study proposes that the primary role of gldA is to remove toxic dihydroxyacetone by converting it into glycerol.

Role of GldA in dihydroxyacetone and methylglyoxal metabolism of Escherichia coli K12. Publishing Authors By Initials

KP Subedi,I Kim,J Kim,B Min,C Park,KP Subedi,I Kim,J Kim,B Min,C Park,

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Role of GldA in dihydroxyacetone and methylglyoxal metabolism of Escherichia coli K12. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: FEMS microbiology letters

VOLUME: 279

Page Numbers: 180-7

Journal Abbreviation: FEMS Microbiol. Lett.

ISSN: 0378-1097

DAY: 20

MONTH: 12

YEAR: 2007

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LANGUAGE: eng

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Grant and Affiliation Information for Role of GldA in dihydroxyacetone and methylglyoxal metabolism of Escherichia coli K12.

AFFILIATION: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yusong-Ku, Taejon, Korea.

Country: England

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MEDLINETA: FEMS Microbiol Lett

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