Role of ERK 1/2 signaling in neuronal differentiation of cultured embryonic stem cells.

Role of ERK 1/2 signaling in neuronal differentiation of cultured embryonic stem cells. Research Abstract Details 

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Role of ERK 1/2 signaling in neuronal differentiation of cultured embryonic stem cells. Abstract Text:

Zegui Li,Michelle Hedrick Theus,Ling Wei,

Embryonic stem (ES) cells represent an ideal source for cell engraftment in the damaged central nervous system (CNS). Understanding key signals that control ES cell differentiation may improve cell type-specific differentiation that is suitable for transplantation therapy. We tested the hypothesis that extracellular signal-regulated kinase (ERK) 1/2 phosphorylation is an early signaling event required for the neuronal differentiation of ES cells. Cultured mouse ES cells were treated with an all-trans-retinoic-acid (RA) protocol to generate neurally induced progenitor cells. Western blot analysis showed a dramatic increase in ERK 1/2 phosphorylation (p-ERK 1/2) 1-5 days after RA induction, which was attenuated in the presence of the p-ERK 1/2-specific inhibitor UO126. Phospho-ERK 1/2 inhibition significantly reduced the number of NeuN-positive cells and the expression of associated cytoskeletal proteins. In differentiating ES cells, there was increased nuclear translocation of STAT3 and decreased protein expression levels of GDNF, BDNF and NGF. STAT3 translocation was attenuated by UO126. Finally, caspase-3 activation was observed in the presence of UO126, suggesting that the ERK pathway also contributes to the survival of differentiating ES cells. These data indicate that ERK 1/2 phosphorylation is a key event required for early neuronal differentiation and survival of ES cells.

Role of ERK 1/2 signaling in neuronal differentiation of cultured embryonic stem cells. Publishing Authors By Initials

Z Li,MH Theus,L Wei,

For similar organic chemicals: hydrocarbons: hydrocarbons, acyclic: alkenes: polyenes: carotenoids: retinoids: vitamin a: tretinoin research abstracts see: organic chemicals: hydrocarbons: hydrocarbons, acyclic: alkenes: polyenes: carotenoids: retinoids: vitamin a: tretinoin research

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Role of ERK 1/2 signaling in neuronal differentiation of cultured embryonic stem cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Development, growth & differentiation

VOLUME: 48

Page Numbers: 513-23

Journal Abbreviation: Dev. Growth Differ.

ISSN: 0012-1592

DAY: 3

MONTH: Oct

YEAR: 2006

Role of ERK 1/2 signaling in neuronal differentiation of cultured embryonic stem cells. Information

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LANGUAGE: eng

NlmUniqueID: 356504

Role of ERK 1/2 signaling in neuronal differentiation of cultured embryonic stem cells. Keywords Mesh Terms:

KEYWORDS: Tretinoin

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Role of ERK 1/2 signaling in neuronal differentiation of cultured embryonic stem cells. Information

Substance Name: Mitogen-Activated Protein Kinase 3

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Role of ERK 1/2 signaling in neuronal differentiation of cultured embryonic stem cells.

AFFILIATION: Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, South Carolina 29425, USA.

Country: Japan

AGENCY: United States NINDS

GRANT: NS045810

ACRONYM: NS

MEDLINETA: Dev Growth Differ

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