Role of calcium mobilization in the regulation of spontaneous transient outward currents in porcine coronary artery myocytes.

Role of calcium mobilization in the regulation of spontaneous transient outward currents in porcine coronary artery myocytes. Research Abstract Details 

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Role of calcium mobilization in the regulation of spontaneous transient outward currents in porcine coronary artery myocytes. Abstract Text:

Pengyun Li,Xiaorong Zeng,Yan Yang,Fang Cai,Zhifei Liu,Miaoling Li,Jie Pei,Wen Zhou,PengYun Li,XiaoRong Zeng,Yan Yang,Fang Cai,ZhiFei Liu,MiaoLing Li,Jie Pei,Wen Zhou,PengYun Li,XiaoRong Zeng,Yan Yang,Fang Cai,ZhiFei Liu,MiaoLing Li,Jie Pei,Wen Zhou,

The purpose of the present study was to further study the characteristics and regulation of spontaneous transient outward currents (STOCs) in freshly isolated porcine coronary artery smooth muscle cells (ASMCs). STOCs were recorded using the perforated whole-cell patch-clamp configuration. STOCs were voltage-dependent and superimposed stochastically onto whole-cell Ca(2+)-activated-K(+) (BK(Ca)) currents. Charybdotoxin (ChTX, 200 nmol/L), a selective blocker of BK(Ca) channels, completely inhibited STOCs within 10 min. STOCs activity was greatly suppressed when extracellular Ca(2+) concentration decreased from 1.8 mmol/L to 200 nmol/L, further removal of Ca(2+) abolished STOCs activity. Ca(2+) ionophore A23187 (10 mumol/L) increased STOCs activity significantly. Verapamil (20 mumol/L) and CdCl(2) (200 mumol/L), two kinds of organic L-type voltage-dependent Ca(2+) channels (L-VDCCs) antagonists, had little effect on STOCs. In addition, the ryanodine receptors (RyRs) agonist caffeine (5 mmol/L) significantly activated STOCs. Application of ryanodine (50 mumol/L) to block RyRs abolished STOCs, subsequent washout of ryanodine or application of caffeine failed to reproduce STOCs activity. Inhibition of inositol 1,4,5-trisphosphate receptors (IP(3)Rs) by 2APB (40 mumol/L) greatly suppressed the activity of STOCs, application of caffeine (5 mmol/L) in the presence of 2APB caused a burst of outward currents followed by inhibition of STOCs. These results suggest that STOCs in porcine coronary ASMCs are mediated by BK(Ca) channels. Extracellular Ca(2+) is essential for STOCs activity, while Ca(2+) entry through L-VDCCs has little effect on STOCs. Intracellular Ca(2+) release induced by RyRs is responsible for the regulation of STOCs, whereas IP3Rs might also be involved.

Role of calcium mobilization in the regulation of spontaneous transient outward currents in porcine coronary artery myocytes. Publishing Authors By Initials

P Li,X Zeng,Y Yang,F Cai,Z Liu,M Li,J Pei,W Zhou,P Li,X Zeng,Y Yang,F Cai,Z Liu,M Li,J Pei,W Zhou,P Li,X Zeng,Y Yang,F Cai,Z Liu,M Li,J Pei,W Zhou,

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Role of calcium mobilization in the regulation of spontaneous transient outward currents in porcine coronary artery myocytes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Science in China. Series C, Life sciences / Chines

VOLUME: 50

Page Numbers: 660-8

Journal Abbreviation: Sci. China, C, Life Sci.

ISSN: 1006-9305

DAY: 19

MONTH: Oct

YEAR: 2007

Role of calcium mobilization in the regulation of spontaneous transient outward currents in porcine coronary artery myocytes. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Role of calcium mobilization in the regulation of spontaneous transient outward currents in porcine coronary artery myocytes.

AFFILIATION: Institute of Myocardial Electrophysiology, Luzhou Medical College, Luzhou, 646000, China, zengxr8818@163.com.

Country: China

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MEDLINETA: Sci China C Life Sci

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