Role of beta1 integrins in adhesion of canine mastocytoma cells to extracellular matrix proteins.

Role of beta1 integrins in adhesion of canine mastocytoma cells to extracellular matrix proteins. Research Abstract Details 

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Role of beta1 integrins in adhesion of canine mastocytoma cells to extracellular matrix proteins. Abstract Text:

Tomoko Takahashi,Emi Ohashi,Takayuki Nakagawa,Manabu Mochizuki,Ryohei Nishimura,Nobuo Sasaki,Tomoko Takahashi,Emi Ohashi,Takayuki Nakagawa,Manabu Mochizuki,Ryohei Nishimura,Nobuo Sasaki,

The interactions of tumor cells with the extracellular matrix (ECM) are a crucial step in invasion and metastasis. Integrins are adhesive molecules forming heterodimers with alpha and beta subunits that play a definitive role in these interactions. In this study, mastocytoma (mast cell tumor: MCT) cell-ECM interaction was investigated using 3 canine MCT cell lines: CM-MC (originating from cutaneous MCT), VI-MC (originating from intestinal MCT), and CoMS (originating from oral MCT). Flow cytometric analysis showed that all cells highly expressed the integrin beta1 and alpha1 through alpha5 subunits and that they moderately expressed the alpha6 subunit. In adhesion studies, CoMS weakly but spontaneously adhered to fibronectin (FN), which was enhanced by phorbol ester (TPA), while CM-MC and VI-MC required cell activation by TPA to adhere to FN. Anti-beta1 and alpha5 integrin antibodies strongly inhibited cell adhesion to FN in CM-MC and CoMS and moderately inhibited cell adhesion in VI-MC. Only VI-MC adhered to laminin (LN) under activation by TPA. Anti-beta1 integrin antibodies strongly inhibited cell adhesion to LN, but all anti-alpha integrin antibodies failed to inhibit cell adhesion to LN. No cells adhered to collagen types I and IV. Canine MCT cells from different origins expressed similar integrin patterns; however, there were some differences in adhesive behavior in response to various ECM proteins and activating stimuli.

Role of beta1 integrins in adhesion of canine mastocytoma cells to extracellular matrix proteins. Publishing Authors By Initials

T Takahashi,E Ohashi,T Nakagawa,M Mochizuki,R Nishimura,N Sasaki,T Takahashi,E Ohashi,T Nakagawa,M Mochizuki,R Nishimura,N Sasaki,

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Role of beta1 integrins in adhesion of canine mastocytoma cells to extracellular matrix proteins. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of veterinary medical science / the Ja

VOLUME: 69

Page Numbers: 495-9

Journal Abbreviation: J. Vet. Med. Sci.

ISSN: 0916-7250

DAY: 6

MONTH: May

YEAR: 2007

Role of beta1 integrins in adhesion of canine mastocytoma cells to extracellular matrix proteins. Information

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LANGUAGE: eng

NlmUniqueID: 9105360

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AFFILIATION: Laboratory of Comprehensive Veterinary Clinical Studies, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Kameino, Fujisawa, Kanagawa, Japan.

Country: Japan

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MEDLINETA: J Vet Med Sci

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