Role of Bacillus anthracis spore structures in macrophage cytokine responses.

Role of Bacillus anthracis spore structures in macrophage cytokine responses. Research Abstract Details 

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Role of Bacillus anthracis spore structures in macrophage cytokine responses. Abstract Text:

Subhendu Basu,Tae Jin Kang,Wilbur H Chen,Matthew J Fenton,Les Baillie,Steve Hibbs,Alan S Cross,

The innate immune response of macrophages (Mphi) to spores, the environmentally acquired form of Bacillus anthracis, is poorly characterized. We therefore examined the early Mphi cytokine response to B. anthracis spores, before germination. Mphi were exposed to bacilli and spores of Sterne strain 34F2 and its congenic nongerminating mutant (DeltagerH), and cytokine expression was measured by real-time PCR and an enzyme-linked immunosorbent assay. The exosporium spore layer was retained (exo+) or removed by sonication (exo-). Spores consistently induced a strong cytokine response, with the exo- spores eliciting a two- to threefold-higher response than exo+ spores. The threshold for interleukin-1beta (IL-1beta) production by wild-type Mphi was significantly lower than that required for tumor necrosis factor alpha expression. Cytokine production was largely dependent on MyD88, suggesting Toll-like receptor involvement; however, the expression of beta interferon in MyD88-/- Mphi suggests involvement of a MyD88-independent pathway. We conclude that (i) the B. anthracis spore is not immunologically inert, (ii) the exosporium masks epitopes recognized by the Mphi, (iii) the Mphi cytokine response to B. anthracis involves multiple pattern recognition receptors and signaling pathways, and (iv) compared to other cytokines, IL-1beta is expressed at a lower spore concentration.

Role of Bacillus anthracis spore structures in macrophage cytokine responses. Publishing Authors By Initials

S Basu,TJ Kang,WH Chen,MJ Fenton,L Baillie,S Hibbs,AS Cross,

For similar peptides: intercellular signaling peptides and proteins: cytokines: monokines: tumor necrosis factor-alpha research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: monokines: tumor necrosis factor-alpha research

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Role of Bacillus anthracis spore structures in macrophage cytokine responses. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Infection and immunity

VOLUME: 75

Page Numbers: 2351-8

Journal Abbreviation:

ISSN: 0019-9567

DAY: 5

MONTH: 03

YEAR: 2007

Role of Bacillus anthracis spore structures in macrophage cytokine responses. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 246127

Role of Bacillus anthracis spore structures in macrophage cytokine responses. Keywords Mesh Terms:

KEYWORDS: Tumor Necrosis Factor-alpha

MESH TERMS: genetics

Chemical & Substance for Abstract: Role of Bacillus anthracis spore structures in macrophage cytokine responses. Information

Substance Name: Tumor Necrosis Factor-alpha

Registry Number: 0

Grant and Affiliation Information for Role of Bacillus anthracis spore structures in macrophage cytokine responses.

AFFILIATION: Center for Vaccine Development, Department of Medicine, University of Maryland, 685 W. Baltimore Street, HSF I-480, Baltimore, MD 21201, USA. sbasu1@medicine.umaryland.edu

Country: United States

AGENCY: United States NIAID

GRANT: U54 AI-057168

ACRONYM: AI

MEDLINETA: Infect Immun

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