Role for Fox-1/Fox-2 in mediating the neuronal pathway of calcitonin/calcitonin gene-related peptide alternative RNA processing.

Role for Fox-1/Fox-2 in mediating the neuronal pathway of calcitonin/calcitonin gene-related peptide alternative RNA processing. Research Abstract Details 

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Role for Fox-1/Fox-2 in mediating the neuronal pathway of calcitonin/calcitonin gene-related peptide alternative RNA processing. Abstract Text:

Hua-Lin Zhou,Andrew P Baraniak,Hua Lou,

Although multiple regulatory elements and protein factors are known to regulate the non-neuronal pathway of alternative processing of the calcitonin/calcitonin gene-related peptide (CGRP) pre-mRNA, the mechanisms controlling the neuron-specific pathway have remained elusive. Here we report the identification of Fox-1 and Fox-2 proteins as novel regulators that mediate the neuron-specific splicing pattern. Fox-1 and Fox-2 proteins function to repress exon 4 inclusion, and this effect depends on two UGCAUG elements surrounding the 3' splice site of the calcitonin-specific exon 4. In neuron-like cells, mutation of a subset of UGCAUG elements promotes the non-neuronal pattern in which exon 4 is included. In HeLa cells, overexpression of Fox-1 or Fox-2 protein decreases exon 4 inclusion. Fox-1 and Fox-2 proteins interact with the UGCAUG elements specifically and regulate splicing by blocking U2AF(65) binding to the 3' splice site upstream of exon 4. We further investigated the inter-relationship between the UGCAUG silencer elements and the previously identified intronic and exonic splicing regulatory elements and found that exon 4 is regulated by an intricate balance of positive and negative regulation. These results define a critical role for Fox-1 and Fox-2 proteins in exon 4 inclusion of calcitonin/CGRP pre-mRNA and establish a regulatory network that controls the fate of exon 4.

Role for Fox-1/Fox-2 in mediating the neuronal pathway of calcitonin/calcitonin gene-related peptide alternative RNA processing. Publishing Authors By Initials

HL Zhou,AP Baraniak,H Lou,

For similar proteins: carrier proteins: rna-binding proteins: ribonucleoproteins research abstracts see: proteins: carrier proteins: rna-binding proteins: ribonucleoproteins research

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Role for Fox-1/Fox-2 in mediating the neuronal pathway of calcitonin/calcitonin gene-related peptide alternative RNA processing. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular and cellular biology

VOLUME: 27

Page Numbers: 830-41

Journal Abbreviation: Mol. Cell. Biol.

ISSN: 0270-7306

DAY: 13

MONTH: 11

YEAR: 2006

Role for Fox-1/Fox-2 in mediating the neuronal pathway of calcitonin/calcitonin gene-related peptide alternative RNA processing. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8109087

Role for Fox-1/Fox-2 in mediating the neuronal pathway of calcitonin/calcitonin gene-related peptide alternative RNA processing. Keywords Mesh Terms:

KEYWORDS: Ribonucleoproteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Role for Fox-1/Fox-2 in mediating the neuronal pathway of calcitonin/calcitonin gene-related peptide alternative RNA processing. Information

Substance Name: Calcitonin Gene-Related Peptide

Registry Number: 83652-28-2

Grant and Affiliation Information for Role for Fox-1/Fox-2 in mediating the neuronal pathway of calcitonin/calcitonin gene-related peptide alternative RNA processing.

AFFILIATION: Department of Genetics, Case Western Reserve University, 10900 Euclid Ave., Cleveland, OH 44106, USA. hxl47@case.edu

Country: United States

AGENCY: United States NIGMS

GRANT: R01 GM 63090

ACRONYM: GM

MEDLINETA: Mol Cell Biol

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