Robust immunohistochemical staining of several classes of proteins in tissues subjected to autolysis.

Robust immunohistochemical staining of several classes of proteins in tissues subjected to autolysis. Research Abstract Details 

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Robust immunohistochemical staining of several classes of proteins in tissues subjected to autolysis. Abstract Text:

Joseph Maleszewski,Jie Lu,Karen Fox-Talbot,Marc K Halushka,

Despite the common use of immunohistochemistry in autopsy tissues, the stability of most proteins over extended time periods is unknown. The robustness of signal for 16 proteins (MMP1, MMP2, MMP3, MMP9, TIMP1, TIMP2, TIMP3, AGER, MSR, SCARB1, OLR1, CD36, LTF, LGALS3, LYZ, and DDOST) and two measures of advanced glycation end products (AGE, CML) was evaluated. Two formalin-fixed, paraffin-embedded human tissue arrays containing 16 tissues each were created to evaluate 48 hr of autolysis in a warm or cold environment. For these classes of proteins, matrix metalloproteinases and their inhibitors, scavenger receptors, and advanced glycation end product receptors, we saw no systematic diminution of signal intensity during a period of 24 hr. Analysis was performed by two independent observers and confirmed for a subset of proteins by digital analysis and Western blotting. We conclude that these classes of proteins degrade slowly and faithfully maintain their immunohistochemistry characteristics over at least a 24-hr time interval in devitalized tissues. This study supports the use of autopsy tissues with short postmortem intervals for immunohistochemical studies for diseases such as diabetic vascular disease, cancer, Alzheimer's disease, atherosclerosis, and other pathological states. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials.

Robust immunohistochemical staining of several classes of proteins in tissues subjected to autolysis. Publishing Authors By Initials

J Maleszewski,J Lu,K Fox-Talbot,MK Halushka,

For similar proteins: tissue inhibitor of metalloproteinases research abstracts see: proteins: tissue inhibitor of metalloproteinases research

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Robust immunohistochemical staining of several classes of proteins in tissues subjected to autolysis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The journal of histochemistry and cytochemistry :

VOLUME: 55

Page Numbers: 597-606

Journal Abbreviation: J. Histochem. Cytochem.

ISSN: 0022-1554

DAY: 20

MONTH: 02

YEAR: 2007

Robust immunohistochemical staining of several classes of proteins in tissues subjected to autolysis. Information

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LANGUAGE: eng

NlmUniqueID: 9815334

Robust immunohistochemical staining of several classes of proteins in tissues subjected to autolysis. Keywords Mesh Terms:

KEYWORDS: Tissue Inhibitor of Metalloproteinases

MESH TERMS: metabolism

Chemical & Substance for Abstract: Robust immunohistochemical staining of several classes of proteins in tissues subjected to autolysis. Information

Substance Name: Matrix Metalloproteinases

Registry Number: EC 3.4.24.-

Grant and Affiliation Information for Robust immunohistochemical staining of several classes of proteins in tissues subjected to autolysis.

AFFILIATION: Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21205, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: P01 HL-056091

ACRONYM: HL

MEDLINETA: J Histochem Cytochem

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