RNAPol-ChIP analysis of transcription from FSHD-linked tandem repeats and satellite DNA.

RNAPol-ChIP analysis of transcription from FSHD-linked tandem repeats and satellite DNA. Research Abstract Details 

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RNAPol-ChIP analysis of transcription from FSHD-linked tandem repeats and satellite DNA. Abstract Text:

Vassilios Alexiadis,Mary E Ballestas,Cecilia Sanchez,Sara Winokur,Vettaikorumakankav Vedanarayanan,Mary Warren,Melanie Ehrlich,

RNA interference (RNAi) is implicated in maintaining tandem DNA arrays as constitutive heterochromatin. We used chromatin immunoprecipitation with antibodies to RNA polymerase II (RNAPol-ChIP) to test for transcription of the following repeat arrays in human cells: subtelomeric D4Z4, pericentromeric satellite 2, and centromeric satellite alpha. D4Z4 has a promoter-like sequence upstream of an ORF in its 3.3-kb repeat unit. A short D4Z4 array at 4q35 is linked to facioscapulohumeral muscular dystrophy (FSHD). By RNAPol-ChIP and RT-PCR, little or no transcription of D4Z4 was detected in FSHD and normal myoblasts; lymphoblasts from an FSHD patient, a control, and a patient with D4Z4 hypomethylation due to mutation of DNMT3B (ICF syndrome); and normal or cancer tissues. However, RNAPol-ChIP assays indicated transcription of D4Z4 in a chromosome 4-containing human-mouse somatic cell hybrid. ChIP and RT-PCR showed satellite DNA transcription in some cancers and lymphoblastoid cell lines, although only at a low level. Given the evidence for the involvement of RNAi in satellite DNA heterochromatinization, it is surprising that, at most, a very small fraction of satellite DNA was associated with RNA Pol II. In addition, our results do not support the previously hypothesized disease-linked differential transcription of D4Z4 sequences in short, FSHD-linked arrays.

RNAPol-ChIP analysis of transcription from FSHD-linked tandem repeats and satellite DNA. Publishing Authors By Initials

V Alexiadis,ME Ballestas,C Sanchez,S Winokur,V Vedanarayanan,M Warren,M Ehrlich,

For similar genetic processes: gene expression: transcription, genetic research abstracts see: genetic processes: gene expression: transcription, genetic research

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RNAPol-ChIP analysis of transcription from FSHD-linked tandem repeats and satellite DNA. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochimica et biophysica acta

VOLUME: 1769

Page Numbers: 29-40

Journal Abbreviation: Biochim. Biophys. Acta

ISSN: 0006-3002

DAY: 22

MONTH: 11

YEAR: 2006

RNAPol-ChIP analysis of transcription from FSHD-linked tandem repeats and satellite DNA. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 217513

RNAPol-ChIP analysis of transcription from FSHD-linked tandem repeats and satellite DNA. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: genetics

Chemical & Substance for Abstract: RNAPol-ChIP analysis of transcription from FSHD-linked tandem repeats and satellite DNA. Information

Substance Name: RNA Polymerase II

Registry Number: EC 2.7.7.-

Grant and Affiliation Information for RNAPol-ChIP analysis of transcription from FSHD-linked tandem repeats and satellite DNA.

AFFILIATION: Genpathway, Inc., San Diego, CA 92121, USA.

Country: Netherlands

AGENCY: United States NINDS

GRANT: R01 NS048859-02S1

ACRONYM: NS

MEDLINETA: Biochim Biophys Acta

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