RNA aptamers targeted to domain II of hepatitis C virus IRES that bind to its apical loop region.

RNA aptamers targeted to domain II of hepatitis C virus IRES that bind to its apical loop region. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

RNA aptamers targeted to domain II of hepatitis C virus IRES that bind to its apical loop region. Abstract Text:

Kunio Kikuchi,Takuya Umehara,Kotaro Fukuda,Joonsung Hwang,Atsushi Kuno,Tsunemi Hasegawa,Satoshi Nishikawa,

The internal ribosome entry site (IRES) is important for translation of hepatitis C virus (HCV) mRNA and has a unique RNA structure containing conserved domains I to IV. To investigate the function of domain II, we selected RNA aptamers that bind to domain II of HCV IRES by applying a simple and convenient selection method using a hybridized tag for fixing domain II RNA on magnetic beads instead of synthesizing long RNA. In addition, we employed surface plasmon resonance (SPR) technology to measure the binding affinity of each generation and to obtain detailed kinetic constants. The selected aptamers have a consensus sequence, 5'-UAUGGCU-3', which is complementary to the apical loop of domain II. The loop-loop interaction between the consensus sequence and domain II was confirmed by mutagenesis and nuclease mapping analyses. Binding affinities were dependent on the local structure containing the conserved sequence. The aptamers could inhibit IRES-dependent translation.

RNA aptamers targeted to domain II of hepatitis C virus IRES that bind to its apical loop region. Publishing Authors By Initials

K Kikuchi,T Umehara,K Fukuda,J Hwang,A Kuno,T Hasegawa,S Nishikawa,

For similar nucleic acids, nucleotides, and nucleosides: nucleic acids: rna: rna, viral research abstracts see: nucleic acids, nucleotides, and nucleosides: nucleic acids: rna: rna, viral research

PUBMED ID PMID:

MEDLINE DATE:

RNA aptamers targeted to domain II of hepatitis C virus IRES that bind to its apical loop region. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 133

Page Numbers: 263-70

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Mar

YEAR: 2003

RNA aptamers targeted to domain II of hepatitis C virus IRES that bind to its apical loop region. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

RNA aptamers targeted to domain II of hepatitis C virus IRES that bind to its apical loop region. Keywords Mesh Terms:

KEYWORDS: RNA, Viral

MESH TERMS: metabolism

Chemical & Substance for Abstract: RNA aptamers targeted to domain II of hepatitis C virus IRES that bind to its apical loop region. Information

Substance Name: RNA, Viral

Registry Number: 0

Grant and Affiliation Information for RNA aptamers targeted to domain II of hepatitis C virus IRES that bind to its apical loop region.

AFFILIATION: Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan.

Country: Japan

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

RNA aptamers targeted to domain II of hepatitis C virus IRES that bind to its apical loop region Related Publications

• ARID3B induces malignant transformation of mouse embryonic fibroblasts and is strongly associated with malignant neuroblastoma.
• Altered EphA5 mRNA expression in rat brain with a single methamphetamine treatment.
• Association of IL-8-inducing strains of diffusely adherent Escherichia coli with sporadic diarrheal patients with less than 5 years of age.
• Changing the amino acid specificity of yeast tyrosyl-tRNA synthetase by genetic engineering.
• Compositional changes in RNA, DNA and proteins for bacterial adaptation to higher and lower temperatures.
• Crystallization and preliminary X-ray analysis of the HA3 component of Clostridium botulinum type C progenitor toxin.
• Detection of pathogenic yeasts from processed fresh edible sea urchins sold in a fish market.
• Detection of structural changes of RNA aptamer containing GGA repeats under the ionic condition using the microchip electrophoresis.
• Developmental changes in pulpal sensory innervation of rat incisors and molars shown on a single injection of the fluorescent dye AM1-43.
• Effect of pH on the taste of alkaline electrolyzed water.
• Effects of schizophrenomimetics on the expression of the CCN1 (CYR 61) gene encoding a matricellular protein in the infant and adult neocortex of the mouse and rat.
• Elastic properties of active muscle--on the rebound?
• Enhancement of Shiga Toxin Production in Enterohemorrhagic Escherichia coli Serotype O157:H7 by DNase Colicins.
• Enhancement of esophageal carcinogenesis in acid reflux model rats treated with ascorbic acid and sodium nitrite in combination with or without initiation.
• Evaluation of the Levels of Specific IgE against Cryptococcus diffluens and Cryptococcus liquefaciens in Patients with Atopic Dermatitis.
• Hsp-based tumor vaccines: state-of-the-art and future directions.
• Inclusion kinetics of a nucleotide into a cyclodextrin cavity by means of ultrasonic relaxation.
• Influence of lactic acid bacteria on longevity of Caenorhabditis elegans and host defense against salmonella enterica serovar enteritidis.
• Long-term cure in surgery for extrarectal pelvic recurrence of rectal cancer.
• Molecular cloning of delta-4, a new mouse and human Notch ligand.
• NK314, a novel topoisomerase II inhibitor, induces rapid DNA double-strand breaks and exhibits superior antitumor effects against tumors resistant to other topoisomerase II inhibitors.
• Phylogenetic position of a whale-fall lancelet (Cephalochordata) inferred from whole mitochondrial genome sequences.
• RNA aptamers targeted to domain II of hepatitis C virus IRES that bind to its apical loop region.
• Role of Calcium Messenger Systems in ACTH-induced Cortisol Production in Bovine Adrenal Fasciculo-reticularis Cells.
• Structure/function analysis of an RNA aptamer for hepatitis C virus NS3 protease.
• The journal of gene medicine Japanese society of gene therapy young investigator award 2007.
• Theoretical study of above-threshold dissociation on diatomic molecules by using nonresonant intense laser pulses.
• Variants of eae and stx genes of atypical enteropathogenic Escherichia coli and non-O157 Shiga toxin-producing Escherichia coli from calves.
• Wang-Landau molecular dynamics technique to search for low-energy conformational space of proteins.
• [Prevalence and evaluation of secondary hypertension]