Rice shaker potassium channel OsKAT1 confers tolerance to salinity stress on yeast and rice cells.

Rice shaker potassium channel OsKAT1 confers tolerance to salinity stress on yeast and rice cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Rice shaker potassium channel OsKAT1 confers tolerance to salinity stress on yeast and rice cells. Abstract Text:

Toshihiro Obata,Hiroko K Kitamoto,Atsuko Nakamura,Atsunori Fukuda,Yoshiyuki Tanaka,

We screened a rice (Oryza sativa L. 'Nipponbare') full-length cDNA expression library through functional complementation in yeast (Saccharomyces cerevisiae) to find novel cation transporters involved in salt tolerance. We found that expression of a cDNA clone, encoding the rice homolog of Shaker family K(+) channel KAT1 (OsKAT1), suppressed the salt-sensitive phenotype of yeast strain G19 (Deltaena1-4), which lacks a major component of Na(+) efflux. It also suppressed a K(+)-transport-defective phenotype of yeast strain CY162 (Deltatrk1Deltatrk2), suggesting the enhancement of K(+) uptake by OsKAT1. By the expression of OsKAT1, the K(+) contents of salt-stressed G19 cells increased during the exponential growth phase. At the linear phase, however, OsKAT1-expressing G19 cells accumulated less Na(+) than nonexpressing cells, but almost the same K(+). The cellular Na(+) to K(+) ratio of OsKAT1-expressing G19 cells remained lower than nonexpressing cells under saline conditions. Rice cells overexpressing OsKAT1 also showed enhanced salt tolerance and increased cellular K(+) content. These functions of OsKAT1 are likely to be common among Shaker K(+) channels because OsAKT1 and Arabidopsis (Arabidopsis thaliana) KAT1 were able to complement the salt-sensitive phenotype of G19 as well as OsKAT1. The expression of OsKAT1 was restricted to internodes and rachides of wild-type rice, whereas other Shaker family genes were expressed in various organs. These results suggest that OsKAT1 is involved in salt tolerance of rice in cooperation with other K(+) channels by participating in maintenance of cytosolic cation homeostasis during salt stress and thus protects cells from Na(+).

Rice shaker potassium channel OsKAT1 confers tolerance to salinity stress on yeast and rice cells. Publishing Authors By Initials

T Obata,HK Kitamoto,A Nakamura,A Fukuda,Y Tanaka,

For similar chlorides: sodium chloride research abstracts see: chlorides: sodium chloride research

PUBMED ID PMID:

MEDLINE DATE:

Rice shaker potassium channel OsKAT1 confers tolerance to salinity stress on yeast and rice cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Plant physiology

VOLUME: 144

Page Numbers: 1978-85

Journal Abbreviation: Plant Physiol.

ISSN: 0032-0889

DAY: 22

MONTH: 06

YEAR: 2007

Rice shaker potassium channel OsKAT1 confers tolerance to salinity stress on yeast and rice cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 401224

Rice shaker potassium channel OsKAT1 confers tolerance to salinity stress on yeast and rice cells. Keywords Mesh Terms:

KEYWORDS: Sodium Chloride

MESH TERMS: metabolism

Chemical & Substance for Abstract: Rice shaker potassium channel OsKAT1 confers tolerance to salinity stress on yeast and rice cells. Information

Substance Name: Sodium Chloride

Registry Number: 7647-14-5

Grant and Affiliation Information for Rice shaker potassium channel OsKAT1 confers tolerance to salinity stress on yeast and rice cells.

AFFILIATION: Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Plant Physiol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Rice shaker potassium channel OsKAT1 confers tolerance to salinity stress on yeast and rice cells Related Publications

• A clonal analysis of neural progenitors during axolotl spinal cord regeneration reveals evidence for both spatially restricted and multipotent progenitors.
• Ankle skeletal injury predictions using anisotropic inelastic constitutive model of cortical bone taking into account damage evolution.
• Antiviral substances from the root of paeonia species.
• Application of a fine thread beam to the structure analysis of a hemihedrally twinned crystal of hydroxylamine oxidoreductase.
• Application of in vitro alternative methods to ecotoxicology.
• Association of Activities of Daily Living and Indices of Mental Status with Subsequent 20-year All-Cause Mortality in an Elderly Japanese Population.
• Circadian and light-induced transcription of clock gene Per1 depends on histone acetylation and deacetylation.
• Concentration of copper and a copper-EDTA complex at the pH junction formed in soil by an electrokinetic remediation process.
• Degradation of Ginseng Saponins under Mild Acidic Conditions.
• Diuretic action of paeonol.
• Effects Of Habitual Exercise And Gene Polymorphism On Quantitative Ultrasound Parameters In Japanese Elderly: 1291: Board #54 May 30 11:00 AM -12:30 PM.
• Ferritization treatment of copper in soil by electrokinetic remediation.
• Focal hepatic lesions mimicking cavernous hemangioma supplied by the portal vein.
• Further Study on Dammarane-Saponins of Leaves and Stems of American Ginseng, Panax quinquefolium.
• Improvement of the resonant cavity applicator for brain tumor hyperthermia - Computer simulation results -.
• Improvement of the resonant cavity applicator for brain tumor hyperthermia - Experimental heating results -.
• Influence of nutrient deficiency caused by host developmental arrest on the growth and development of a koinobiont parasitoid.
• Inhibition of NF-kappa B transcriptional activity by alpha-tocopheryl succinate.
• Optimal replication of poliovirus within cells.
• Panax ginseng: Relation between Age of Plant and Content of Ginsenosides.
• Proposal of a high-field superconducting wiggler for a slow positron source at SPring-8.
• Purification and Characterization of Antiviral Substance from the Bud of Syzygium aromatica.
• Seasonal variation of saponins, sucurose and monosaccharides in cultivated ginseng roots.
• Solubilization of saponins of bupleuri radix with ginseng saponins: cooperative effect of dammarane saponins.
• Studies on the Tissue Culture of Stevia rebaudiana and Its Components; (II)1. Induction of Shoot Primordia.
• Temperature distributions of developed needle type applicator.
• Tissue plasminogen activator and thrombin generation measurements using the Thrombinoscope.
• [Characteristics of polycyclic aromatic hydrocarbons (PAHS) on airborne particulates in Beijing]
• [Comparing identification of 3 kinds of flos chrysanthemi on surface morphology]
• [Drought tolerance of transgenic sweet potato expressing both Cu/Zn superoxide dismutase and ascorbate peroxidase]