Reversal effect of BM-cyclin 1 on multidrug resistance in C-A120 cells.

Reversal effect of BM-cyclin 1 on multidrug resistance in C-A120 cells. Research Abstract Details 

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Reversal effect of BM-cyclin 1 on multidrug resistance in C-A120 cells. Abstract Text:

Lin Wang,Jian Sun,Yong-Qiang Li,Zhe-Sheng Chen,Shin-Ichi Akiyama,Li-Jian Xian,

In this study, multidrug-resistant human epidermoid C-A120 cells and the sensitive parental KB cells were used as experimental models. BM-cyclin 1, a traditional antimycoplasma drug, was tested to explore the reversal effect of multidrug resistance and its mechanisms in these cell lines. The MTT analysis showed that BM-cyclin 1 could reverse multidrug resistance effectively in C-A120 cells; the sensitivity of C-A120 cells to adriamycin, etoposide and cisplatin was enhanced by 6.0, 8.2 and 1.7 times, respectively. Immunoblotting analysis and reverse transcription-polymerase chain reaction were used to study the BM-cyclin 1-induced changes in topoisomerase IIalpha. The results showed that the expression of topoisomerase IIalpha in treated C-A120 cells increased significantly. Topoisomerase II catalytic activity increased by 30% compared with the untreated cells, as measured by decatenation of kinetopolast DNA. Immunoblotting analysis also indicated the transcription factor levels of specificity: those of protein 1 (Sp1) and nuclear factor-YA increased after treatment with BM-cyclin 1, whereas the mRNA and protein expression of multidrug resistance protein 2 was significantly downregulated. These results demonstrated that BM-cyclin 1 could effectively reverse the multidrug resistance of C-A120 cells by increasing the expression of topoisomerase IIalpha and by suppressing the expression of multidrug resistance protein 2, strongly suggesting that BM-cyclin 1 is a potential multidrug resistance reversal agent.

Reversal effect of BM-cyclin 1 on multidrug resistance in C-A120 cells. Publishing Authors By Initials

L Wang,J Sun,YQ Li,ZS Chen,S Akiyama,LJ Xian,

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Reversal effect of BM-cyclin 1 on multidrug resistance in C-A120 cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Anti-cancer drugs

VOLUME: 18

Page Numbers: 1015-21

Journal Abbreviation: Anticancer Drugs

ISSN: 0959-4973

DAY: 30

MONTH: Oct

YEAR: 2007

Reversal effect of BM-cyclin 1 on multidrug resistance in C-A120 cells. Information

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LANGUAGE: eng

NlmUniqueID: 9100823

Reversal effect of BM-cyclin 1 on multidrug resistance in C-A120 cells. Keywords Mesh Terms:

KEYWORDS: Reverse Transcriptase Polymerase Chain R

MESH TERMS: biosynthesis

Chemical & Substance for Abstract: Reversal effect of BM-cyclin 1 on multidrug resistance in C-A120 cells. Information

Substance Name: DNA topoisomerase II alpha

Registry Number: EC 5.99.1.3

Grant and Affiliation Information for Reversal effect of BM-cyclin 1 on multidrug resistance in C-A120 cells.

AFFILIATION: State Key Laboratory of Oncology in Southern China.

Country: England

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MEDLINETA: Anticancer Drugs

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