Retroviral Vectors Encoding a Reverse Transcription-activated Transgene Efficiently Limit Expression of the Gene to Target Cells.

Retroviral Vectors Encoding a Reverse Transcription-activated Transgene Efficiently Limit Expression of the Gene to Target Cells. Research Abstract Details 

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Retroviral Vectors Encoding a Reverse Transcription-activated Transgene Efficiently Limit Expression of the Gene to Target Cells. Abstract Text:

John Douglas Burke,John C Morris,John Douglas Burke,John C Morris,

Recombinant retroviral vectors are indispensable tools for the study of gene function and for therapeutic gene transfer owing to their ability to transfer and stably express foreign genes in target cells. A limitation of these vectors, however, is the difficulty in generating stable vector producer cell (VPC) lines when the vectors encode cytotoxic proteins. We developed a series of Moloney murine leukemia virus-based vectors encoding a reverse transcription-activated transgene. These vectors preclude gene expression in the producer cells, yet allow lines for transgene expression in target cells. The vectors were generated by cloning the gene of interest in reverse orientation either just upstream of the viral 3' long terminal repeat (LTR) or in the U3 region of the 3'LTR. An exogenous promoter was inserted, also in reverse orientation, at the R-U5 border of the viral 5'LTR. Upon transduction of target cells, the inserted promoter is copied to the 3'LTR during reverse transcription of the vector genomic RNA, where it then drives transgene expression. We tested this system using a green fluorescent protein (GFP) gene and the SV40 promoter. Reverse transcription-activated retroviral vectors may allow for the generation of stable retroviral VPC lines encoding cytotoxic or inhibitory genes.Molecular Therapy (2007) 15, 552-559. doi:10.1038/sj.mt.6300062; published online 26 December 2006.

Retroviral Vectors Encoding a Reverse Transcription-activated Transgene Efficiently Limit Expression of the Gene to Target Cells. Publishing Authors By Initials

JD Burke,JC Morris,JD Burke,JC Morris,

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Retroviral Vectors Encoding a Reverse Transcription-activated Transgene Efficiently Limit Expression of the Gene to Target Cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Molecular therapy : the journal of the American So

VOLUME: 15

Page Numbers: 552-9

Journal Abbreviation: Mol. Ther.

ISSN: 1525-0016

DAY: 26

MONTH: 12

YEAR: 2006

Retroviral Vectors Encoding a Reverse Transcription-activated Transgene Efficiently Limit Expression of the Gene to Target Cells. Information

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LANGUAGE: eng

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AFFILIATION: 1Metabolism Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.

Country: United States

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MEDLINETA: Mol Ther

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