Retention behavior of ginsenosides on a poly(vinyl alcohol)-bonded stationary phase in hydrophilic interaction chromatography.

Retention behavior of ginsenosides on a poly(vinyl alcohol)-bonded stationary phase in hydrophilic interaction chromatography. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Retention behavior of ginsenosides on a poly(vinyl alcohol)-bonded stationary phase in hydrophilic interaction chromatography. Abstract Text:

Noel S Quiming,Nerissa L Denola,Azamjon B Soliev,Yoshihiro Saito,Kiyokatsu Jinno,Noel S Quiming,Nerissa L Denola,Azamjon B Soliev,Yoshihiro Saito,Kiyokatsu Jinno,

The influences of the organic component of the mobile phase and the column temperature on the retention of ginsenosides on a poly(vinyl alcohol) (PVA) bonded stationary phase operated under hydrophilic interaction chromatographic mode were investigated. The retention of the ginsenosides was found to increase with increasing amount of acetonitrile (MeCN) in the mobile phase, which is typical of hydrophilic interaction chromatographic behavior. It was also found that the retention of the analytes was highly affected by the type of the organic modifier used. Aqueous MeCN (75-90%) gave the most satisfactory retention and separation of ginsenosides Rf, Rg1, Rd, Re, Rc, Rb2 and Rb1 compared with aqueous methanol, isopropyl alcohol or tetrahydrofuran at the same composition levels. The effects of the different types of organic modifiers on the retention of the analytes were attributed to their solvent strength and hydrogen-bond accepting/donating properties. The effect of temperature on the retention of ginsenoside on the PVA-bonded phase was assessed by constructing van't Hoff plots for two temperature ranges: subambient (273-293 K) and ambient-elevated (298-333 K) temperatures. van't Hoff plots for all analytes were linear at the two temperature intervals; however, the slopes of the lines corresponding to ginsenosides Rg1 and Re were completely different from those for the rest of the analytes especially in the subambient temperature range. Enthalpy-entropy compensation (EEC) studies were conducted to verify the difference in thermodynamics observed for ginsenosides Rg1 and Re compared with the other analytes. EEC plots showed that Rf, Rd, Rc, Rb2 and Rb1 were possibly retained by the same retention mechanism, which was completely different from that of Rg1 and Re at subambient temperatures. Retention prediction models were derived using multiple linear regression to identify solute attributes that affected the retention of the analytes on the PVA-bonded phase. The mathematical models derived revealed that the number of hydrogen-bond donors and the ovality of the molecules are important molecular properties that govern the retention of the compounds on the chromatographic system.

Retention behavior of ginsenosides on a poly(vinyl alcohol)-bonded stationary phase in hydrophilic interaction chromatography. Publishing Authors By Initials

NS Quiming,NL Denola,AB Soliev,Y Saito,K Jinno,NS Quiming,NL Denola,AB Soliev,Y Saito,K Jinno,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Retention behavior of ginsenosides on a poly(vinyl alcohol)-bonded stationary phase in hydrophilic interaction chromatography. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Analytical and bioanalytical chemistry

VOLUME: 389

Page Numbers: 1477-88

Journal Abbreviation:

ISSN: 1618-2642

DAY: 6

MONTH: 09

YEAR: 2007

Retention behavior of ginsenosides on a poly(vinyl alcohol)-bonded stationary phase in hydrophilic interaction chromatography. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101134327

Retention behavior of ginsenosides on a poly(vinyl alcohol)-bonded stationary phase in hydrophilic interaction chromatography. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Retention behavior of ginsenosides on a poly(vinyl alcohol)-bonded stationary phase in hydrophilic interaction chromatography. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Retention behavior of ginsenosides on a poly(vinyl alcohol)-bonded stationary phase in hydrophilic interaction chromatography.

AFFILIATION: School of Materials Science, Toyohashi University of Technology, Toyohashi 441-8580, Japan.

Country: Germany

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Anal Bioanal Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Retention behavior of ginsenosides on a polyvinyl alcohol-bonded stationary phase in hydrophilic interaction chromatography Related Publications

• A HhaI/BstUI polymorphism in a novel gene at human chromosome 11p15.5.
• Development of retention prediction models for adrenoreceptor agonists and antagonists on a polyvinyl alcohol-bonded stationary phase in hydrophilic interaction chromatography.
• Effects of alcohols on CE enantioseparation of basic drugs with native gamma-CD as chiral selector.
• Fast Separation and Quantification of C60 and C70 Fullerenes Using Thermostated Micro Thin-layer Chromatography.
• Fiber-packed needle for dynamic extraction of aromatic compounds.
• Interaction of native alpha-cyclodextrin, beta-cyclodextrin and gamma-cyclodextrin and their hydroxypropyl derivatives with selected organic low molecular mass compounds at elevated and subambient temperature under RP-HPLC conditions.
• Multiple linear regression and artificial neural network retention prediction models for ginsenosides on a polyamine-bonded stationary phase in hydrophilic interaction chromatography.
• Retention Prediction Modeling of Ginsenosides on a Polyvinyl Alcohol-bonded Stationary Phase at Subambient Temperatures Using Multiple Linear Regression and Artificial Neural Network.
• Retention behavior of ginsenosides on a poly(vinyl alcohol)-bonded stationary phase in hydrophilic interaction chromatography.
• Simultaneous enantioseparation and sensitivity enhancement of basic drugs using large-volume sample stacking.
• [Myocardial structure and left ventricular function in aortic valvular diseases]