Restriction fragment length polymorphism in the mitochondrial DNA of cloned cattle.

Restriction fragment length polymorphism in the mitochondrial DNA of cloned cattle. Research Abstract Details 

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Restriction fragment length polymorphism in the mitochondrial DNA of cloned cattle. Abstract Text:

Y Plante,S M Schmutz,K D Lang,

The clonal origin of 4 Holstein bulls was determined by hybridization experiments with 2 different minisatellite probes, and all 4 animals showed identical genomic DNA fingerprints, hence confirming monozygosity. Extra-chromosomal differences were observed among these 4 Holstein bulls. Mitochondrial DNA restriction fragment length polymorphisms with restriction endonucleases Avall and Hhal sites were found, and these polymorphisms can be explained by the loss of a single site for each of these 2 enzymes. Since mitochondrial DNA are maternally transmitted, all 4 bulls would produce genetically equivalent spermatozoa and offspring. The combination of embryo cloning and specific cytoplasmic markers would provide an ideal system for the study of maternal cytoplasmic effects on quantitative traits.

Restriction fragment length polymorphism in the mitochondrial DNA of cloned cattle. Publishing Authors By Initials

Y Plante,SM Schmutz,KD Lang,

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Restriction fragment length polymorphism in the mitochondrial DNA of cloned cattle. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 38

Page Numbers: 897-904

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: Nov

YEAR: 1992

Restriction fragment length polymorphism in the mitochondrial DNA of cloned cattle. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Restriction fragment length polymorphism in the mitochondrial DNA of cloned cattle.

AFFILIATION: DNA Laboratory Saskatchewan Research Council Saskatoon, SK S7N 2X0 Canada; Department of Animal and Poultry Science University of Saskatchewan, Saskatoon, SK S7N 0W0 Canada.

Country: United States

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MEDLINETA: Theriogenology

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