[Restriction endonuclease Sst 12I from a strain of Streptomyces sp. recognizing the nucleotide sequence 5'-CTGCAG-3']
[Restriction endonuclease Sst 12I from a strain of Streptomyces sp. recognizing the nucleotide sequence 5'-CTGCAG-3'] Research Abstract Details
[Restriction endonuclease Sst 12I from a strain of Streptomyces sp. recognizing the nucleotide sequence 5'-CTGCAG-3'] Abstract Text:
A new restriction endonuclease Sst12I belonging to the II type and recognizing the sequence 5'-CTGCAG-3' was isolated from the bacterial strain Streptomyces sp. St-12. The enzyme hydrolyzes DNA between adenine and guanine residues; thus, it is a true isoschizomer of restrictase PstI. In contrast to PstI, the restriction endonuclease Sst12I hydrolyses DNA both at 37 degrees and 55 degrees C and remains active after long-term storage.
[Restriction endonuclease Sst 12I from a strain of Streptomyces sp. recognizing the nucleotide sequence 5'-CTGCAG-3'] Publishing Authors By Initials
[Restriction endonuclease Sst 12I from a strain of Streptomyces sp. recognizing the nucleotide sequence 5'-CTGCAG-3'] Journal Published:
PUBLICATION TYPE: Journal Article
Journal: Prikladnaia biokhimiia i mikrobiologiia
VOLUME: 38
Page Numbers: 25-8
Journal Abbreviation: Prikl. Biokhim. Mikrobiol.
ISSN: 0555-1099
DAY: 29
MONTH: 08
YEAR: 2008
[Restriction endonuclease Sst 12I from a strain of Streptomyces sp. recognizing the nucleotide sequence 5'-CTGCAG-3'] Information
Number of References:
LANGUAGE: rus
NlmUniqueID: 23416
[Restriction endonuclease Sst 12I from a strain of Streptomyces sp. recognizing the nucleotide sequence 5'-CTGCAG-3'] Keywords Mesh Terms:
KEYWORDS: Temperature
MESH TERMS: enzymology
Chemical & Substance for Abstract: [Restriction endonuclease Sst 12I from a strain of Streptomyces sp. recognizing the nucleotide sequence 5'-CTGCAG-3'] Information
Substance Name: Deoxyribonucleases, Type II Site-Specifi
Registry Number: EC 3.1.21.4
Grant and Affiliation Information for [Restriction endonuclease Sst 12I from a strain of Streptomyces sp. recognizing the nucleotide sequence 5'-CTGCAG-3']
AFFILIATION: State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk Oblast, 630559 Russia.
Country: Russia
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MEDLINETA: Prikl Biokhim Mikrobiol
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